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A simple assay system for examination of the inhibitory potential in vivo of decoy RNAs, ribozymes and other drugs by measuring the Tat-mediated transcription of a fusion gene composed of the long terminal repeat of HIV-1 and a gene for luciferase.
Koseki S, Ohkawa J, Yamamoto R, Takebe Y, Taira K. Koseki S, et al. Among authors: taira k. J Control Release. 1998 Apr 30;53(1-3):159-73. doi: 10.1016/s0168-3659(97)00250-2. J Control Release. 1998. PMID: 9741924
Significantly higher activity of a cytoplasmic hammerhead ribozyme than a corresponding nuclear counterpart: engineered tRNAs with an extended 3' end can be exported efficiently and specifically to the cytoplasm in mammalian cells.
Kuwabara T, Warashina M, Koseki S, Sano M, Ohkawa J, Nakayama K, Taira K. Kuwabara T, et al. Among authors: taira k. Nucleic Acids Res. 2001 Jul 1;29(13):2780-8. doi: 10.1093/nar/29.13.2780. Nucleic Acids Res. 2001. PMID: 11433023 Free PMC article.
Construction of a tRNA-embedded-ribozyme trimming plasmid.
Yuyama N, Ohkawa J, Inokuchi Y, Shirai M, Sato A, Nishikawa S, Taira K. Yuyama N, et al. Among authors: taira k. Biochem Biophys Res Commun. 1992 Aug 14;186(3):1271-9. doi: 10.1016/s0006-291x(05)81543-8. Biochem Biophys Res Commun. 1992. PMID: 1510661
[Inhibition of HIV replication by ribozyme strategy].
Ohkawa J, Taira K. Ohkawa J, et al. Among authors: taira k. Tanpakushitsu Kakusan Koso. 1995 Jul;40(10):1421-9. Tanpakushitsu Kakusan Koso. 1995. PMID: 7568943 Review. Japanese. No abstract available.
A multifunctional expression vector for an anti-HIV-1 ribozyme that produces a 5'- and 3'-trimmed trans-acting ribozyme, targeted against HIV-1 RNA, and cis-acting ribozymes that are designed to bind to and thereby sequester trans-activator proteins such as Tat and Rev.
Yuyama N, Ohkawa J, Koguma T, Shirai M, Taira K. Yuyama N, et al. Among authors: taira k. Nucleic Acids Res. 1994 Nov 25;22(23):5060-7. doi: 10.1093/nar/22.23.5060. Nucleic Acids Res. 1994. PMID: 7800500 Free PMC article.
895 results