Identification of the RSX interactome in a marsupial shows functional coherence with the Xist interactome during X inactivation

Genome Biol. 2024 May 23;25(1):134. doi: 10.1186/s13059-024-03280-0.

Abstract

The marsupial specific RSX lncRNA is the functional analogue of the eutherian specific XIST, which coordinates X chromosome inactivation. We characterized the RSX interactome in a marsupial representative (the opossum Monodelphis domestica), identifying 135 proteins, of which 54 had orthologues in the XIST interactome. Both interactomes were enriched for biological pathways related to RNA processing, regulation of translation, and epigenetic transcriptional silencing. This represents a remarkable example showcasing the functional coherence of independently evolved lncRNAs in distantly related mammalian lineages.

Keywords: RSX protein interactome; Convergent evolution; X chromosome inactivation.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Monodelphis / genetics
  • Monodelphis / metabolism
  • RNA, Long Noncoding* / genetics
  • RNA, Long Noncoding* / metabolism
  • X Chromosome Inactivation*

Substances

  • RNA, Long Noncoding
  • XIST non-coding RNA