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Denaturing purifications demonstrate that PRC2 and other widely reported chromatin proteins do not appear to bind directly to RNA in vivo.
Mol Cell. 2024 Apr 4;84(7):1271-1289.e12. doi: 10.1016/j.molcel.2024.01.026. Epub 2024 Feb 21.
Mol Cell. 2024.
PMID: 38387462
SPIDR: a highly multiplexed method for mapping RNA-protein interactions uncovers a potential mechanism for selective translational suppression upon cellular stress.
Wolin E, Guo JK, Blanco MR, Perez AA, Goronzy IN, Abdou AA, Gorhe D, Guttman M, Jovanovic M.
Wolin E, et al.
bioRxiv [Preprint]. 2023 Jun 7:2023.06.05.543769. doi: 10.1101/2023.06.05.543769.
bioRxiv. 2023.
PMID: 37333139
Free PMC article.
Preprint.
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ChIP-DIP maps binding of hundreds of proteins to DNA simultaneously and identifies diverse gene regulatory elements.
Perez AA, Goronzy IN, Blanco MR, Yeh BT, Guo JK, Lopes CS, Ettlin O, Burr A, Guttman M.
Perez AA, et al.
Nat Genet. 2024 Dec;56(12):2827-2841. doi: 10.1038/s41588-024-02000-5. Epub 2024 Nov 25.
Nat Genet. 2024.
PMID: 39587360
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