Abstract
Klassevirus is a proposed new genus of picornavirus that has been associated with pediatric diarrhea. In this study, we used recombinant klassevirus 3C protease as the capture antigen for an indirect serological enzyme-linked immunosorbent assay (ELISA). Four of six klassevirus reverse transcription (RT)-PCR-positive individuals demonstrated seroconversion against the 3C protease, suggesting that klassevirus infection and replication occur in humans. Additional screening of 353 samples from an age-banded serological cohort from two St. Louis hospitals indicated a seroprevalence of 6.8%.
Publication types
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Research Support, N.I.H., Extramural
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Research Support, Non-U.S. Gov't
MeSH terms
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Adolescent
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Adult
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Aged
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Antigens, Viral*
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Child
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Child, Preschool
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Enzyme-Linked Immunosorbent Assay / methods
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Humans
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Infant
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Infant, Newborn
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Middle Aged
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Molecular Sequence Data
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Picornaviridae / immunology*
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Picornaviridae Infections / diagnosis*
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RNA, Viral / genetics
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Reverse Transcriptase Polymerase Chain Reaction
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Sequence Analysis, DNA
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Serologic Tests / methods
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Viral Nonstructural Proteins
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Virology / methods*
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Young Adult
Substances
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Antigens, Viral
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RNA, Viral
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Viral Nonstructural Proteins
Associated data
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GENBANK/GU992865
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GENBANK/GU992866
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GENBANK/GU992867
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GENBANK/GU992868
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GENBANK/GU992869
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GENBANK/GU992870