Exploring the genetic diversity and population structure of scarlet eggplant germplasm from Rwanda through iPBS-retrotransposon markers

Mol Biol Rep. 2021 Sep;48(9):6323-6333. doi: 10.1007/s11033-021-06626-0. Epub 2021 Aug 12.

Abstract

Background: Scarlet eggplant (Solanum aethiopicum gr. gilo) is a part of African indigenous vegetables and acknowledged as a source of variations in the breeding of Brinjal. Since its genetic diversity is still largely unexplored, therefore genetic diversity and population structure of this plant were investigated in this study.

Methods and results: Scarlet eggplant germplasm made of fifty-two accessions originated from two districts of Rwanda was assessed by employing the iPBS-retrotransposon markers system. Twelve most polymorphic primers were employed for molecular characterization and they yielded 329 total bands whereupon 85.03% were polymorphic. The recorded mean polymorphism information content was 0.363 and other diversity indices such as; mean the effective number of alleles, mean Shannon's information index and gene diversity with the following values; 1.298, 0.300 and 0.187 respectively. A superior level of diversity was noticed among accessions from Musanze district. The model-based structure, neighbor-joining, and principal coordinate analysis (PCoA) gathered scarlet germplasm in a divergence manner to their collection district. Analysis of molecular variance (AMOVA) displayed that the utmost variations (81%) in scarlet eggplant germplasm are resulting in differences within populations.

Conclusions: The extensive diversity of scarlet eggplant in Rwanda might be used to form the base and genetic resource of an exhaustive breeding program of this economically important African indigenous vegetable. For instance, accessions MZE53 and GKE11 might be proposed as parent candidates due to their high relative genetic distance (0.6781).

Keywords: African indigenous vegetable; Eggplant wild relative; Germplasm characterization; Orphan crop; Solanum aethiopicum.

MeSH terms

  • Alleles
  • DNA Primers / genetics*
  • DNA, Plant / genetics
  • DNA, Plant / isolation & purification
  • Plant Breeding
  • Polymorphism, Genetic*
  • Retroelements / genetics*
  • Reverse Transcriptase Polymerase Chain Reaction / methods*
  • Rwanda
  • Seeds / genetics*
  • Solanum / genetics*
  • Solanum melongena / genetics*
  • Terminal Repeat Sequences / genetics

Substances

  • DNA Primers
  • DNA, Plant
  • Retroelements