On-Site Sensitive Colorimetry for Free Cyanide by Using Ion-Pair Solid-Phase Extraction with Sedimentable Dispersed Particulates and Mobile Photography Box

We propose a sensitive and simple colorimetric method using dispersed particle extraction for the highly sensitive detection of free cyanide in water samples. The method involves the direct capture of the color-producing compound by dispersed microparticles in a sample vessel containing colorimetric reagents and an adsorbent. The color of the microparticles that have naturally settled to the bottom of the microtube can be directly measured by visual observation or image analysis. A mobile photography box (MPB) suitable for use with a smartphone was developed to ensure reproducibility in the lighting environment during image acquisition. The MPB was then used to develop a highly sensitive analytical method for cyanide. This newly developed method allows direct measurement of the color tone of the target component collected on particles by irradiating light from below and photographing it perpendicularly. The method offers excellent portability, sensitivity, and reproducibility and is less affected by interfering color components. When applied to cyanide analysis, the detection limit reached 0.005 mg/L and measurements could be completed within 10 min, significantly shorter than the conventional absorbance spectrophotometric method, which requires 30 min. Thus, this method achieves highly sensitive cyanide analysis.