Improving CRISPR-Cas-mediated RNA targeting and gene editing using SPLCV replicon-based expression vectors in Nicotiana benthamiana

Plant Biotechnol J. 2020 Oct;18(10):1993-1995. doi: 10.1111/pbi.13384. Epub 2020 Apr 28.

¹ Jiangsu Key Laboratory of Phylogenomics and Comparative Genomics, School of Life Sciences, Jiangsu Normal University, Xuzhou, Jiangsu, China.
² Xuzhou Institute of Agricultural Sciences in Jiangsu Xuhuai District, Xuzhou, Jiangsu, China.
³ Institute of Food Crops, Provincial Key Laboratory of Agrobiology, Jiangsu Academy of Agricultural Sciences, Nanjing, Jiangsu, China.

^# Contributed equally.

No abstract available

Keywords: Nicotiana benthamiana; LbCas12a; LwaCas13a; RNA targeting and gene editing efficiency; SpCas9; deconstructed SPLCV strategy.

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