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The Human Neonatal Skin Fibroblast, an Available Cell Source for Tissue Production and Transplantation, Exhibits Low Risk of Immunogenicity In Vitro.
Magne B, Ferland K, Savard É, Barbier MA, Morissette A, Larouche D, Beaudoin-Cloutier C, Germain L. Magne B, et al. Int J Mol Sci. 2024 Jun 26;25(13):6965. doi: 10.3390/ijms25136965. Int J Mol Sci. 2024. PMID: 39000078 Free PMC article.
In this study, we sought to investigate whether fibroblasts derived from either adult or neonatal human skin tissues could induce different immune responses toward phagocytosis and T cell activation using in vitro co-culture models. Our results indicate that both phagocyto …
In this study, we sought to investigate whether fibroblasts derived from either adult or neonatal human skin tissues could induce different …
Cannabinoid receptors are expressed in equine synovium and upregulated with synovitis.
Miagkoff L, Girard CA, St-Jean G, Richard H, Beauchamp G, Laverty S. Miagkoff L, et al. Equine Vet J. 2023 Jul;55(4):681-695. doi: 10.1111/evj.13860. Epub 2022 Aug 13. Equine Vet J. 2023. PMID: 35836386
RESULTS: CB1 was expressed in synovial intimal cells in all specimens studied whereas CB2 expression was identified in 94%. Both receptors were also expressed in the subintimal blood vessel walls. ...Conversely, CB1 expression significantly decreased (p = 0.03) with increa …
RESULTS: CB1 was expressed in synovial intimal cells in all specimens studied whereas CB2 expression was identified in 94%. Both rece …
A Newly Developed Chemically Defined Serum-Free Medium Suitable for Human Primary Keratinocyte Culture and Tissue Engineering Applications.
Ghio SC, Barbier MA, Doucet EJ, Debbah I, Safoine M, Le-Bel G, Cartier A, Jolibois E, Morissette A, Larouche D, Fradette J, Guérin SL, Garnier A, Germain L. Ghio SC, et al. Int J Mol Sci. 2023 Jan 17;24(3):1821. doi: 10.3390/ijms24031821. Int J Mol Sci. 2023. PMID: 36768144 Free PMC article.
We also show that keratin-19-positive epithelial stem cells are retained through serial passaging in Surge SFM cultures. Transcriptomic analyses suggest that gene expression is similar between keratinocytes cultured with either Surge SFM or the conventional serum-containin …
We also show that keratin-19-positive epithelial stem cells are retained through serial passaging in Surge SFM cultures. Transcriptom …
An in vitro model for discovery of osteoclast specific biomarkers towards identification of racehorses at risk for catastrophic fractures.
Malek G, Richard H, Beauchamp G, Laverty S. Malek G, et al. Equine Vet J. 2023 May;55(3):534-550. doi: 10.1111/evj.13600. Epub 2022 Jun 19. Equine Vet J. 2023. PMID: 35616632
STUDY DESIGN: In vitro experiments. METHODS: Haematopoietic stem cells, from five equine sternal bone marrow aspirates, were differentiated into osteoclasts and cultured either alone or on equine bone slices, with or without a pro-inflammatory stimulus (IL-1beta or LPS). . …
STUDY DESIGN: In vitro experiments. METHODS: Haematopoietic stem cells, from five equine sternal bone marrow aspirates, were differen …
In Vitro Prevascularization of Self-Assembled Human Bone-Like Tissues and Preclinical Assessment Using a Rat Calvarial Bone Defect Model.
Kawecki F, Galbraith T, Clafshenkel WP, Fortin M, Auger FA, Fradette J. Kawecki F, et al. Materials (Basel). 2021 Apr 17;14(8):2023. doi: 10.3390/ma14082023. Materials (Basel). 2021. PMID: 33920607 Free PMC article.
In vitro prevascularization has the potential to address the challenge of maintaining cell viability at the core of engineered constructs, such as bone substitutes, and to improve the survival of tissue grafts by allowing quicker anastomosis to the host microvasculature. T …
In vitro prevascularization has the potential to address the challenge of maintaining cell viability at the core of engineered constr …