In Vivo Silencing of Genes Coding for dTip60 Chromatin Remodeling Complex Subunits Affects Polytene Chromosome Organization and Proper Development in Drosophila melanogaster

Int J Mol Sci. 2021 Apr 26;22(9):4525. doi: 10.3390/ijms22094525.

Abstract

Chromatin organization is developmentally regulated by epigenetic changes mediated by histone-modifying enzymes and chromatin remodeling complexes. In Drosophila melanogaster, the Tip60 chromatin remodeling complex (dTip60) play roles in chromatin regulation, which are shared by evolutionarily-related complexes identified in animal and plants. Recently, it was found that most subunits previously assigned to the dTip60 complex are shared by two related complexes, DOM-A.C and DOM-B.C, defined by DOM-A and DOM-B isoforms, respectively. In this work, we combined classical genetics, cell biology, and reverse genetics approaches to further investigate the biological roles played during Drosophila melanogaster development by a number of subunits originally assigned to the dTip60 complex.

Keywords: Drosophila melanogaster; chromatin remodeling; epigenetic silencing; polytene chromosomes; position effect variegation.

MeSH terms

  • Animals
  • Chromatin / genetics*
  • Chromatin Assembly and Disassembly / genetics*
  • Drosophila Proteins / genetics*
  • Drosophila Proteins / metabolism
  • Drosophila melanogaster / genetics
  • Epigenesis, Genetic / genetics
  • Histone Acetyltransferases / genetics*
  • Histone Acetyltransferases / metabolism
  • Histones / metabolism
  • Polytene Chromosomes / genetics
  • Polytene Chromosomes / metabolism
  • Transcription Factors / genetics
  • Transcription Factors / metabolism

Substances

  • Chromatin
  • Drosophila Proteins
  • Histones
  • Transcription Factors
  • dom protein, Drosophila
  • Histone Acetyltransferases
  • Tip60 protein, Drosophila