$\beta$II-spectrin is the generally expressed member of the $\beta$-spectrin family of elongated polypeptides that form micrometer-scale networks associated with plasma membranes. We addressed in vivo functions of $\beta$II-spectrin in neurons by knockout of $\beta$II-spectrin in mouse neural progenitors. $\beta$II-spectrin deficiency caused severe defects in long-range axonal connectivity and axonal degeneration. $\beta$II-spectrin- null neurons exhibited reduced axon growth, loss of actin-spectrinbased periodic membrane skeleton, and impaired bidirectional axonal transport of synaptic cargo. We found that $\beta$II-spectrin associates with KIF3A, KIF5B, KIF1A, and dynactin, implicating spectrin in the coupling of motors and synaptic cargo. $\beta$II-spectrin required phosphoinositide lipid binding to promote axonal transport and restore axon growth. Knockout of ankyrin-B (AnkB), a $\beta$II-spectrin partner, primarily impaired retrograde organelle transport, while double knockout of $\beta$II-spectrin and AnkB nearly eliminated transport. Thus, $\beta$II-spectrin promotes both axon growth and axon stability through establishing the actin- spectrin-based membrane-associated periodic skeleton as well as enabling axonal transport of synaptic cargo.
%0 Journal Article
%1 Lorenzo2019
%A Lorenzo, Damaris N.
%A Badea, Alexandra
%A Zhou, Ruobo
%A Mohler, Peter J.
%A Zhuang, Xiaowei
%A Bennett, Vann
%D 2019
%I National Academy of Sciences
%J Proceedings of the National Academy of Sciences of the United States of America
%K axon b2sp cytoskeleton growth microscopy neuro storm transport
%N 31
%P 15686--15695
%R 10.1073/pnas.1820649116
%T $\beta$iI-spectrin promotes mouse brain connectivity through stabilizing axonal plasma membranes and enabling axonal organelle transport
%V 116
%X $\beta$II-spectrin is the generally expressed member of the $\beta$-spectrin family of elongated polypeptides that form micrometer-scale networks associated with plasma membranes. We addressed in vivo functions of $\beta$II-spectrin in neurons by knockout of $\beta$II-spectrin in mouse neural progenitors. $\beta$II-spectrin deficiency caused severe defects in long-range axonal connectivity and axonal degeneration. $\beta$II-spectrin- null neurons exhibited reduced axon growth, loss of actin-spectrinbased periodic membrane skeleton, and impaired bidirectional axonal transport of synaptic cargo. We found that $\beta$II-spectrin associates with KIF3A, KIF5B, KIF1A, and dynactin, implicating spectrin in the coupling of motors and synaptic cargo. $\beta$II-spectrin required phosphoinositide lipid binding to promote axonal transport and restore axon growth. Knockout of ankyrin-B (AnkB), a $\beta$II-spectrin partner, primarily impaired retrograde organelle transport, while double knockout of $\beta$II-spectrin and AnkB nearly eliminated transport. Thus, $\beta$II-spectrin promotes both axon growth and axon stability through establishing the actin- spectrin-based membrane-associated periodic skeleton as well as enabling axonal transport of synaptic cargo.
@article{Lorenzo2019,
abstract = {$\beta$II-spectrin is the generally expressed member of the $\beta$-spectrin family of elongated polypeptides that form micrometer-scale networks associated with plasma membranes. We addressed in vivo functions of $\beta$II-spectrin in neurons by knockout of $\beta$II-spectrin in mouse neural progenitors. $\beta$II-spectrin deficiency caused severe defects in long-range axonal connectivity and axonal degeneration. $\beta$II-spectrin- null neurons exhibited reduced axon growth, loss of actin-spectrinbased periodic membrane skeleton, and impaired bidirectional axonal transport of synaptic cargo. We found that $\beta$II-spectrin associates with KIF3A, KIF5B, KIF1A, and dynactin, implicating spectrin in the coupling of motors and synaptic cargo. $\beta$II-spectrin required phosphoinositide lipid binding to promote axonal transport and restore axon growth. Knockout of ankyrin-B (AnkB), a $\beta$II-spectrin partner, primarily impaired retrograde organelle transport, while double knockout of $\beta$II-spectrin and AnkB nearly eliminated transport. Thus, $\beta$II-spectrin promotes both axon growth and axon stability through establishing the actin- spectrin-based membrane-associated periodic skeleton as well as enabling axonal transport of synaptic cargo.},
added-at = {2020-03-23T21:12:34.000+0100},
author = {Lorenzo, Damaris N. and Badea, Alexandra and Zhou, Ruobo and Mohler, Peter J. and Zhuang, Xiaowei and Bennett, Vann},
biburl = {https://www.bibsonomy.org/bibtex/24b562fdfe1a16ed1faf2a9dd9ba01268/kfriedl},
doi = {10.1073/pnas.1820649116},
file = {:C$\backslash$:/Users/Karoline/AppData/Local/Mendeley Ltd./Mendeley Desktop/Downloaded/Lorenzo et al. - 2019 - $\beta$iI-spectrin promotes mouse brain connectivity through stabilizing axonal plasma membranes and enabling axonal o.pdf:pdf},
interhash = {47eef1efd0e217657d37d42ea7598203},
intrahash = {4b562fdfe1a16ed1faf2a9dd9ba01268},
issn = {10916490},
journal = {Proceedings of the National Academy of Sciences of the United States of America},
keywords = {axon b2sp cytoskeleton growth microscopy neuro storm transport},
month = jul,
number = 31,
pages = {15686--15695},
publisher = {National Academy of Sciences},
timestamp = {2020-04-07T11:45:35.000+0200},
title = {{$\beta$iI-spectrin promotes mouse brain connectivity through stabilizing axonal plasma membranes and enabling axonal organelle transport}},
volume = 116,
year = 2019
}