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Database contains
251540 sequences
from 368 species

IMGT/LIGM-DB sequence

Version:
1.2.11
Database release:
LIGMDB_V12
Citing IMGT/LIGM-DB:
Giudicelli, V., Duroux P., Ginestoux C., Folch G., Jabado-Michaloud J., Chaume D., Lefranc M.-P., Nucleic Acids Res., 34:D781-D784 (2006). PMID:16381979 Abstract Full PDF

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MK308859 - Annotation

Sections
Accession number MK308859
Sequence version 1
Secondary accession numbers
IMGT annotation level by annotators
Definition Homo sapiens immunoglobulin heavy chain variable region (IGHV3-21) mRNA, partial cds.
Species Homo sapiens (human)
Taxonomy cellular organisms; Eukaryota; Opisthokonta; Metazoa; Eumetazoa; Bilateria; Deuterostomia; Chordata; Craniata; Vertebrata; Gnathostomata; Teleostomi; Euteleostomi; Sarcopterygii; Dipnotetrapodomorpha; Tetrapoda; Amniota; Mammalia; Theria; Eutheria; Boreoeutheria; Euarchontoglires; Primates; Haplorrhini; Simiiformes; Catarrhini; Hominoidea; Hominidae; Homininae; Homo; Homo sapiens
Sequence length 296
IMGT/LIGM-DB dates 18-NOV-2019
  16-JUN-2024 (v. 5)

OBTENTION

Specificities
Disease related

IDENTIFICATION

Molecule type cDNA
Configuration type rearranged
Gene type variable
Molecule entity type L-V-D-J-C-sequence
Functionality type productive
Chain type IG-Heavy
Structure type regular
Location type
Other keywords antigen receptor, Immunoglobulin superfamily (IgSF), immunoglobulin (IG), IMGT reference sequence, IMGT-NC inferred allele

CLASSIFICATION

IMGT Groups IGHV
IMGT Subgroups IGHV3

DESCRIPTION

L-V-D-J-C-SEQUENCE   <1..>296
  clone IGHV3-21*01_S5868
  db_xref taxon:9606
  IMGT_allele IGHV3-21*05
  IMGT_gene IGHV3-21
  IMGT_isolate A007
  IMGT_note IARC committee confirms the allele sequence up to and including nucleotide 319 (according to IMGT numbering).
  IMGT_note Inferred functionality:(F)
  mol_type mRNA
  organism Homo sapiens
  productive IARC committee confirms the allele sequence up to and including nucleotide 319 (according to IMGT numbering).
  protein_id QBX89554.1
V-REGION   1..296
  CDR_length [8.8.2]
  FR_length [25.17.38.X]
  IMGT_allele IGHV3-21*05
  IMGT_gene IGHV3-21
  IMGT_note IARC committee confirms the allele sequence up to and including nucleotide 319 (according to IMGT numbering).
  IMGT_note Inferred functionality:(F)
gaggtgcagctggtggagtctgggggaggcctggtcaagcctggggggtccctgagactctcctgtgcagcctctggattcaccttcagtagctatagcatgaactgggtccgccaggctccagggaaggggctggagtgggtttcatacattagtagtagtagtagttacatatactacgcagactcagtgaagggccgattcaccatctccagagacaacgccaagaactcactgtatctgcaaatgaacagcctgagagccgaggacacggctgtgtattactgtgcgagaga E V Q L V E S G G G L V K P G G S L R L S C A A S G F T F S S Y S M N W V R Q A P G K G L E W V S Y I S S S S S Y I Y Y A D S V K G R F T I S R D N A K N S L Y L Q M N S L R A E D T A V Y Y C A R
R C S W W S L G E A W S S L G G P * D S P V Q P L D S P S V A I A * T G S A R L Q G R G W S G F H T L V V V V V T Y T T Q T Q * R A D S P S P E T T P R T H C I C K * T A * E P R T R L C I T V R E
G A A G G V W G R P G Q A W G V P E T L L C S L W I H L Q * L * H E L G P P G S R E G A G V G F I H * * * * * L H I L R R L S E G P I H H L Q R Q R Q E L T V S A N E Q P E S R G H G C V L L C E R
FR1-IMGT   1..75
  AA_IMGT AA 1 to 26, AA 10 is missing
gaggtgcagctggtggagtctgggggaggcctggtcaagcctggggggtccctgagactctcctgtgcagcctct E V Q L V E S G G G L V K P G G S L R L S C A A S
R C S W W S L G E A W S S L G G P * D S P V Q P
G A A G G V W G R P G Q A W G V P E T L L C S L
1st-CYS   64..66
CDR1-IMGT   76..99
  AA_IMGT AA 27 to 38, AA 31, 32, 33 and 34 are missing
ggattcaccttcagtagctatagc G F T F S S Y S
D S P S V A I
I H L Q * L *
FR2-IMGT   100..150
  AA_IMGT AA 39 to 55
atgaactgggtccgccaggctccagggaaggggctggagtgggtttcatac M N W V R Q A P G K G L E W V S Y
* T G S A R L Q G R G W S G F H
E L G P P G S R E G A G V G F I
CONSERVED-TRP   106..108
CDR2-IMGT   151..174
  AA_IMGT AA 56 to 65, AA 60 and 61 are missing
attagtagtagtagtagttacata I S S S S S Y I
L V V V V V T
* * * * * L H
FR3-IMGT   175..288
  AA_IMGT AA 66 to 104, AA 73 is missing
tactacgcagactcagtgaagggccgattcaccatctccagagacaacgccaagaactcactgtatctgcaaatgaacagcctgagagccgaggacacggctgtgtattactgt Y Y A D S V K G R F T I S R D N A K N S L Y L Q M N S L R A E D T A V Y Y C
T T Q T Q * R A D S P S P E T T P R T H C I C K * T A * E P R T R L C I T
L R R L S E G P I H H L Q R Q R Q E L T V S A N E Q P E S R G H G C V L L
3'V-REGION   286..296
tgtgcgagaga C A R
V R E
C E R
2nd-CYS   286..288
CDR3-IMGT   289..296
  AA_IMGT AA 105 to 117, AA 106, 107, 108, 109, 110, 111, 112, 113, 114, 115 and 116 are missing
gcgagaga A R
R E
E R

Literature references

[1] 1-296
Vazquez Bernat N., Corcoran M., Hardt U., Kaduk M., Phad G.E., Martin M., Karlsson Hedestam G.B.
"High-Quality Library Preparation for NGS-Based Immunoglobulin Germline Gene Inference and Repertoire Expression Analysis"
Front Immunol 10:0-660 (2019)
PUBMED: 31024532
[2] 1-296
Vazquez Bernat N., Corcoran M.M., Hardt U., Kaduk M., Phad G.E., Martin M., Karlsson Hedestam G.B.

Submitted to the ENA/GenBank/DDBJ databases (17-DEC-2018)
Microbiology Tumor and Cell biology, Karolinska Institutet, Solnavagen 9, Biomedicum, C7, Solna, Stockholms Lan 17165, Sweden

Database cross-references

Database Identifiers Relation
ENA SRR9842443
MD5 5eddbfea8b7e7765db1b1d80562c16ec

Sequence

Length : 296 BP
Composition : 71 A; 69 C; 92 G; 64 T; 0 other

     gaggtgcagc tggtggagtc tgggggaggc ctggtcaagc ctggggggtc cctgagactc        60
     tcctgtgcag cctctggatt caccttcagt agctatagca tgaactgggt ccgccaggct       120
     ccagggaagg ggctggagtg ggtttcatac attagtagta gtagtagtta catatactac       180
     gcagactcag tgaagggccg attcaccatc tccagagaca acgccaagaa ctcactgtat       240
     ctgcaaatga acagcctgag agccgaggac acggctgtgt attactgtgc gagaga           296