Maternal Plasma miRNAs as Early Biomarkers of Moderate-to-Late-Preterm Birth
Abstract
:1. Introduction
2. Results
2.1. Gene Abundance
2.2. miRNA Pathway Analysis
3. Discussion
Strengths and Limitations
4. Materials and Methods
4.1. Cohorts
4.2. miRNA Analysis
4.3. Gene Expression Analysis
4.4. miRNA Pathway Analysis
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Auckland | Preterm n = 36 | Term n = 36 | p-Value |
---|---|---|---|
Age (years) * | 32.7 (0.70) | 32.4 (0.73) | 0.83 |
Body Mass Index (kg/m2) * | 24.8 (0.73) | 24.6 (0.58) | 0.87 |
Primigravid * | 25 (69.4%) | 28 (77.8%) | 0.59 |
Socioeconomic index score * | 52.5 (2.20) | 54.2 (2.45) | 0.60 |
Gestation at sampling | 15.6 (0.13) | 15.4 (0.13) | 0.23 |
Gestation at delivery (weeks) | 33.8 (0.37) | 40.1 (0.18) | <0.0001 |
Customised birthweight centile | 54.3 (4.97) | 58.1 (3.60) | 0.54 |
Adelaide | Preterm n = 18 | Term n = 18 | |
Age (years) * | 24.6 (1.39) | 22.9 (1.06) | 0.35 |
Body Mass Index (kg/m2) * | 28.3 (1.56) | 26.6 (1.34) | 0.42 |
Primigravid * | 12 (66.7%) | 16 (88.9%) | 0.23 |
Socioeconomic index score * | 33.4 (2.90) | 31.5 (3.06) | 0.66 |
Gestation at sampling | 15.6 (0.14) | 15.7 (0.12) | 0.97 |
Gestation at delivery (weeks) | 33.8 (0.82) | 40.5 (0.19) | <0.0001 |
Customised birthweight centile | 52.2 (7.07) | 53.7 (6.27) | 0.88 |
Rank | Auckland | Adelaide |
---|---|---|
1 | hsa-miR-451-a | hsa-miR-451-a |
2 | hsa-miR-223-3p | hsa-miR-223-3p |
3 | hsa-let-7a-5p | hsa-let-7a-5p |
4 | hsa-let-126-3p | hsa-let-126-3p |
5 | hsa-miR-23a-3p | hsa-miR-142-3p |
6 | hsa-miR-199a-3p-hsa-miR-199b-3p | hsa-miR-4454-hsa-miR-7975 |
7 | hsa-miR-191-5p | hsa-miR-23a-3p |
8 | hsa-miR-142-3p | hsa-miR-191-5p |
9 | hsa-miR-16-5p | hsa-miR-16-5p |
10 | hsa-miR-7g-5p | hsa-miR-199a-3p-hsa-miR-199b-3p |
11 | hsa-miR-4454-hsa-miR-7975 | hsa-miR-7g-5p |
12 | hsa-miR-15b-5p | hsa-miR-15b-5p |
Gene Pathway | Pathway Target Genes |
---|---|
Cell Cycle | CCND1, CCND2, CDK6, CDKN1A, E2F1, E2F2, MYC, SMC1A, CDC27, SMAD3, WEE1, YWHAH, CCNE2 |
Focal Adhesion | CCND1, BCL2, CCND2, IGF1R, ITGA3, PIK3R1, VAV2, VCL, CRK, CRKL, KDR, LAMC1, VEGFA, AKT1, MAPK1 |
Jak-STAT signaling | CCND1, CCND2, IL6R, MYC, PIK3R1, STAT3, SOCS7, SOCS5, SPRED1, AKT1 |
ErbB signalling | CDKN1A, KRAS, MYC, PIK3R1, ABL2, CRK, CRKL, AKT1, MAPK1 |
Chemokine signaling | CXCL8, KRAS, PIK3R1, STAT3, VAV2, WASL, CHUK, CRK, CRKL, GNB2, FOXO3, AKT1, MAPK1 |
Neurotropin signaling | KRAS, MAP3K1, PIK3R1, CRK, CRKL, RPS6KA3, YWHAH, FOXO3, AKT1, MAPK1 |
mTor signaling | PIK3R1, RPS6KA3, VEGFA, AKT1, MAPK1 |
P53 signaling | CCND1, CCND2, CDK6, CDKN1A, MDM4, CCNE2 |
Adherens junction | IGF1R, VCL, WASL, SMAD3, PTPRJ, MAPK1 |
Fc gamma R-mediated phagocytosis | PIK3R1, VAV2, WASL, CRK, CRKL, AKT1, MAPK1 |
B cell receptor signaling | KRAS, PIK3R1, VAV2, CHUK, AKT1, MAPK1 |
VEGF signaling | KRAS, PIK3R1, KDR, VEGFA, AKT1, MAPK1 |
MAPK signaling | KRAS, MAP3K1, MYC, TAB2, CHUK, CRK, CRKL, FGF2, HSPA1B, RPS6KA3, AKT1, MAPK1 |
Toll-like receptor signaling | IL6, CXCL8, PIK3R1, TAB2, CHUK, MAPK1 |
T cell receptor signaling | KRAS, PIK3R1, VAV2, CHUK, AKT1, MAPK1 |
Fc epsilon RI signaling | KRAS, PIK3R1, VAV2, AKT1, MAPK1 |
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Ramzan, F.; Rong, J.; Roberts, C.T.; O’Sullivan, J.M.; Perry, J.K.; Taylor, R.; McCowan, L.; Vickers, M.H. Maternal Plasma miRNAs as Early Biomarkers of Moderate-to-Late-Preterm Birth. Int. J. Mol. Sci. 2024, 25, 9536. https://doi.org/10.3390/ijms25179536
Ramzan F, Rong J, Roberts CT, O’Sullivan JM, Perry JK, Taylor R, McCowan L, Vickers MH. Maternal Plasma miRNAs as Early Biomarkers of Moderate-to-Late-Preterm Birth. International Journal of Molecular Sciences. 2024; 25(17):9536. https://doi.org/10.3390/ijms25179536
Chicago/Turabian StyleRamzan, Farha, Jing Rong, Claire T. Roberts, Justin M. O’Sullivan, Jo K. Perry, Rennae Taylor, Lesley McCowan, and Mark H. Vickers. 2024. "Maternal Plasma miRNAs as Early Biomarkers of Moderate-to-Late-Preterm Birth" International Journal of Molecular Sciences 25, no. 17: 9536. https://doi.org/10.3390/ijms25179536