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Advances in Electrochemical Sensors for Bioanalysis

A special issue of Sensors (ISSN 1424-8220). This special issue belongs to the section "Biosensors".

Deadline for manuscript submissions: closed (20 July 2024) | Viewed by 1025

Special Issue Editor


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Guest Editor
Department of Chemistry & Chemical Biology, The University of New Mexico, Albuquerque, NM 87131, USA
Interests: analytical chemistry; electrochemistry; biochemistry; biosensors; immunosensors

Special Issue Information

Dear Colleagues,

Electrochemical biosensors have gained significant attention over the years not only due to their inherent sensitivity and specificity but also because of their potential for portability and amenability to miniaturization. Recent progress in the fields of microfluidics and micro/nanofabrication, as well as the development of novel conducting and electroactive polymers, functionalized nanomaterials, bionanoconjugates, and electrodes has paved the way for the wide-ranging application of electrochemical sensors.

The importance of electrochemical sensors for biomonitoring and biomarker analysis in environmental, industrial, agricultural, and the above clinical applications is increasingly being recognized. In clinical settings, electrochemical sensor devices have proven to be specifically helpful for personalized medicine, precision theragnostic, and minimally invasive diagnostic applications. Electrochemical biosensors provide an attractive means of analyzing the content of a biological sample due to the direct conversion of a biological event to an electronic signal. In addition, the design/fabrication of electrodes is a key component in this type of sensor. In recent years, the popularity of nanoscale fabrication, including nanomaterials, composite materials, biopolymers, and conducting polymers for electrochemical biosensors, has been increasing. This Special Issue will focus on recent developments in electrochemical biosensing platforms for bioanalysis. Topics of interest include, but are not limited to, the following:

  • Novel approaches to developing electrochemical biosensors;
  • Biomolecule detection;
  • DNA biosensors and immunosensors;
  • Electrochemical interfaces and systems;
  • Nanostructured materials in electrochemical biosensors;
  • Electrochemical analysis of biological molecules in vitro;
  • In-depth mechanisms and detection principles of biological samples.

Dr. Hoda Ilkhani
Guest Editor

Manuscript Submission Information

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Keywords

  • electrochemical sensors
  • sensor design
  • sensor characterization techniques
  • intelligent processing of sensor materials
  • chemical change monitoring
  • electroanalytical techniques
  • DNA biosensors
  • immunosensors

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Published Papers (1 paper)

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Research

12 pages, 3735 KiB  
Article
Detection of α-Galactosidase A Reaction in Samples Extracted from Dried Blood Spots Using Ion-Sensitive Field Effect Transistors
by Alexander Kuznetsov, Andrey Sheshil, Eugene Smolin, Vitaliy Grudtsov, Dmitriy Ryazantsev, Mark Shustinskiy, Tatiana Tikhonova, Irakli Kitiashvili, Valerii Vechorko and Natalia Komarova
Sensors 2024, 24(11), 3681; https://doi.org/10.3390/s24113681 - 6 Jun 2024
Cited by 1 | Viewed by 701
Abstract
Fabry disease is a lysosomal storage disorder caused by a significant decrease in the activity or absence of the enzyme α-galactosidase A. The diagnostics of Fabry disease during newborn screening are reasonable, due to the availability of enzyme replacement therapy. This paper presents [...] Read more.
Fabry disease is a lysosomal storage disorder caused by a significant decrease in the activity or absence of the enzyme α-galactosidase A. The diagnostics of Fabry disease during newborn screening are reasonable, due to the availability of enzyme replacement therapy. This paper presents an electrochemical method using complementary metal-oxide semiconductor (CMOS)-compatible ion-sensitive field effect transistors (ISFETs) with hafnium oxide-sensitive surfaces for the detection of α-galactosidase A activity in dried blood spot extracts. The capability of ISFETs to detect the reaction catalyzed by α-galactosidase A was demonstrated. The buffer composition was optimized to provide suitable conditions for both enzyme and ISFET performance. The use of ISFET structures as sensor elements allowed for the label-free detection of enzymatic reactions with melibiose, a natural substrate of α-galactosidase A, instead of a synthetic fluorogenic one. ISFET chips were packaged with printed circuit boards and microfluidic reaction chambers to enable long-term signal measurement using a custom device. The packaged sensors were demonstrated to discriminate between normal and inhibited GLA activity in dried blood spots extracts. The described method offers a promising solution for increasing the widespread distribution of newborn screening of Fabry disease. Full article
(This article belongs to the Special Issue Advances in Electrochemical Sensors for Bioanalysis)
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