NES may refer to:
- Nintendo Entertainment System, an 8-bit video game console released by Nintendo in 1985
- Netscape Enterprise Server, the former name of the Sun Java System Web Server
- Neurootological and Equilibriometric Society, a German medical society
- Nuclear export signal, a short amino acid sequence in a protein
- National Election Studies, a wide-ranging and comprehensive academic survey performed after every United States election by the University of Michigan
- Nestin (protein), a human gene and protein
- New English School (Jordan)
- New English School (Kuwait)
- New Economic School, in Russia
- New Economic System, East German economic policy
- News Election Service, one of many names used by Voter News Service
- Night eating syndrome
- Northeast State, a part of the Junior State of America which includes the states of New York, Connecticut, Massachusetts, Vermont, New Hampshire, and Maine
- NHS Education for Scotland, the educational wing of NHS Scotland
- Nashville Electric Service, power provider for Nashville, Tennessee