Zona pellucida sperm-binding protein 3, also known as zona pellucida glycoprotein 3 (Zp-3) or the sperm receptor, is a ZP module-containing protein that in humans is encoded by the ZP3 gene.[5] ZP3 is the glycoprotein in the zona pellucida most important for inducting the acrosome reaction of sperm cells at the beginning of fertilization.[6]

ZP3
Identifiers
AliasesZP3, ZP3A, ZP3B, ZPC, Zp-3, zona pellucida glycoprotein 3 (sperm receptor), zona pellucida glycoprotein 3, OOMD3
External IDsOMIM: 182889; MGI: 99215; HomoloGene: 5178; GeneCards: ZP3; OMA:ZP3 - orthologs
Orthologs
SpeciesHumanMouse
Entrez
Ensembl
UniProt
RefSeq (mRNA)

NM_007155
NM_001110354

NM_011776

RefSeq (protein)

NP_001103824
NP_009086

NP_035906

Location (UCSC)Chr 7: 76.4 – 76.44 MbChr 5: 136.01 – 136.02 Mb
PubMed search[3][4]
Wikidata
View/Edit HumanView/Edit Mouse

Function

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The zona pellucida (ZP) is a specialized extracellular matrix that surrounds the oocyte and early embryo. It is composed of three or four glycoproteins (ZP1-4) with various functions during oogenesis, fertilization and preimplantation development. The protein encoded by this gene is a major structural component of the ZP and functions in primary binding and stimulation of the sperm acrosome reaction. The nascent protein contains a N-terminal signal peptide sequence, a conserved "ZP domain" module, a consensus furin cleavage site (CFCS), a polymerization-blocking external hydrophobic patch (EHP), and a C-terminal transmembrane domain. Cleavage at the CFCS separates the mature protein from the EHP, allowing it to incorporate into nascent ZP filaments. A variation in the last exon of this gene has previously served as the basis for an additional ZP3 locus; however, sequence and literature review reveals that there is only one full-length ZP3 locus in the human genome. Another locus encoding a bipartite transcript designated POMZP3 contains a duplication of the last four exons of ZP3, including the above described variation, and maps closely to this gene.[5]

Orthologs of these genes are found throughout Vertebrata. The western clawed frog appears to have two orthologs, and the sea lamprey has seven.[7]

3D Structure

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X-ray crystallographic studies of the N-terminal half of mammalian ZP3 (PDB: 3D4C, 3D4G, 3EF7, 5OSQ​)[8] as well as its full-length avian homolog (PDB: 3NK3, 3NK4​)[9] revealed that the protein's ZP module consists of two immunoglobulin-like domains, ZP-N and ZP-C. The latter, which contains EHP as well as a ZP3-specific subdomain, interacts with the ZP-N domain of a second molecule to generate an antiparallel homodimeric arrangement required for protein secretion.[9]

Mutations

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The Zona Pellucida (ZP) is a complex matrix of glycoprotein that surrounds the oocyte and plays a crucial role in the attachment of sperm during reproduction. Ultimately, through the facilitation of sperm binding and the initiation of the acrosome reaction, the ZP proteins are essential to reproduction and have an important impact on fertility. Research through the Journal of Cellular and Molecular Medicine conducted experiments to determine the mechanisms surrounding possible mutations to the ZP gene and how they would impact fertility.[10] By performing whole-exome sequencing they isolated a genome that had a mutation in the ZP3 and the ZP1 genes. They then transfected these genes into HeLa cell cultures and ran a variety of tests to isolate the consequences of these mutations. The authors wrote this regarding their results:

“The results indicate that the mutations are involved in the reduced secretion of ZP1 and ZP3 and leading to connection failure of the ZP filaments in vitro. The data suggest a potential that the mutations may be involved in the lacking ZP phenotype, which need to be further investigated in vivo.” (Cao, Qiqi, et al.)

It is clear that that the ZP proteins are crucial to expressing a correct ZP phenotype in humans, in which all of the ZP proteins 1-4 are properly functioning. Without this interface of proper protein function, sperm binding is inhibited, and fertility is compromised.

References

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  1. ^ a b c GRCh38: Ensembl release 89: ENSG00000188372Ensembl, May 2017
  2. ^ a b c GRCm38: Ensembl release 89: ENSMUSG00000004948Ensembl, May 2017
  3. ^ "Human PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
  4. ^ "Mouse PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
  5. ^ a b "Entrez Gene: zona pellucida glycoprotein 3 (sperm receptor)".
  6. ^ Litscher, E. S.; Williams, Z.; Wassarman, P. M. (2009). "Zona pellucida glycoprotein ZP3 and fertilization in mammals". Molecular Reproduction and Development. 76 (10): 933–941. doi:10.1002/mrd.21046. PMID 19504560. S2CID 21186053.
  7. ^ "EggNOG Database | Orthology predictions and functional annotation". eggnogdb.embl.de. Archived from the original on 4 March 2019. Retrieved 5 March 2019.
  8. ^ Monné M, Han L, Schwend T, Burendahl S, Jovine L (2008). "Crystal structure of the ZP-N domain of ZP3 reveals the core fold of animal egg coats". Nature. 456 (7222): 653–7. Bibcode:2008Natur.456..653M. doi:10.1038/nature07599. hdl:11563/8930. PMID 19052627. S2CID 4430083. PDB: 3D4C, 3D4G, 3EF7
  9. ^ a b Han L, Monné M, Okumura H, Schwend T, Cherry AL, Flot D, Matsuda T, Jovine L (2010). "Insights into egg coat assembly and egg-sperm interaction from the X-ray structure of full-length ZP3". Cell. 143 (3): 404–15. doi:10.1016/j.cell.2010.09.041. hdl:11563/8931. PMID 20970175. S2CID 18583237. PDB: 3NK3, 3NK4
  10. ^ Cao, Qiqi; Zhao, Chun; Zhang, Xiaolan; Zhang, Heng; Lu, Qianneng; Wang, Congjing; Hu, Yue; Ling, Xiufeng; Zhang, Junqiang; Huo, Ran (2020-06-22). "Heterozygous mutations in ZP1 and ZP3 cause formation disorder of ZP and female infertility in human". Journal of Cellular and Molecular Medicine. 24 (15): 8557–8566. doi:10.1111/jcmm.15482. ISSN 1582-1838. PMC 7412702. PMID 32573113.

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Further reading

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This article incorporates text from the United States National Library of Medicine, which is in the public domain.


  1. ^ Cao, Qiqi, et al. “Heterozygous Mutations in ZP1 and ZP3 Cause Formation ...” Journal of Cellular and Molecular Medicine, Wiley Online Library, 22 June 2020, onlinelibrary.wiley.com/doi/10.1111/jcmm.15482.