TAS2R1: Difference between revisions
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{{Short description|Member of the 25 known human bitter taste receptors}} |
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{{Infobox_gene}} |
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'''Taste receptor type 2 member 1 (TAS2R1/T2R1)''' is a [[protein]] that in humans is encoded by the ''TAS2R1'' [[gene]].<ref name="pmid10761934">{{cite journal | vauthors = Adler E, Hoon MA, Mueller KL, Chandrashekar J, Ryba NJ, Zuker CS | title = A novel family of mammalian taste receptors | journal = Cell | volume = 100 | issue = 6 | pages = 693–702 | date = Apr 2000 | pmid = 10761934 | doi = 10.1016/S0092-8674(00)80705-9 | s2cid = 14604586 | doi-access = free }}</ref><ref name="pmid10766242">{{cite journal | vauthors = Matsunami H, Montmayeur JP, Buck LB | title = A family of candidate taste receptors in human and mouse | journal = Nature | volume = 404 | issue = 6778 | pages = 601–4 | date = Apr 2000 | pmid = 10766242 | doi = 10.1038/35007072 | bibcode = 2000Natur.404..601M | s2cid = 4336913 }}</ref><ref name="entrez">{{cite web | title = Entrez Gene: TAS2R1 taste receptor, type 2, member 1| url = https://www.ncbi.nlm.nih.gov/sites/entrez?Db=gene&Cmd=ShowDetailView&TermToSearch=50834}}</ref> It belongs to the [[G protein-coupled receptor]] (GPCR) family and is related to [[GPCR family A|class A]]-like GPCRs, they contain 7 transmembrane helix bundles and short [[N-terminus]] loop.<ref name=":0">{{Citation|last1=Di Pizio|first1=Antonella|title=G Protein-Coupled Receptors - Signaling, Trafficking and Regulation|volume=132|date=2016|series=Methods in Cell Biology|pages=401–427|publisher=Elsevier|isbn=978-0-12-803595-5|last2=Levit|first2=Anat|last3=Slutzki|first3=Michal|last4=Behrens|first4=Maik|last5=Karaman|first5=Rafik|last6=Niv|first6=Masha Y.|chapter=Comparing Class a GPCRS to bitter taste receptors |doi=10.1016/bs.mcb.2015.10.005|pmid=26928553}}</ref> Furthermore, TAS2R1 is member of the 25 known human bitter taste receptors, which enable the [[Bitter taste evolution|perception of bitter taste]] in the mouth cavity. Increasing evidence indicates a functional role of TAS2Rs in extra-oral tissues.<ref name=":1">{{Cite journal|last1=Lu|first1=Ping|last2=Zhang|first2=Cheng-Hai|last3=Lifshitz|first3=Lawrence M.|last4=ZhuGe|first4=Ronghua|date=2017-01-04|title=Extraoral bitter taste receptors in health and disease|journal=The Journal of General Physiology|volume=149|issue=2|pages=181–197|doi=10.1085/jgp.201611637|pmid=28053191|issn=0022-1295|pmc=5299619}}</ref> |
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| require_manual_inspection = no |
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| update_protein_box = yes |
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| update_summary = yes |
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| update_citations = yes |
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}} |
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== Expression and function == |
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{{GNF_Protein_box |
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| image = |
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| image_source = |
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| PDB = |
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| Name = Taste receptor, type 2, member 1 |
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| HGNCid = 14909 |
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| Symbol = TAS2R1 |
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| AltSymbols =; MGC126778; MGC126780; T2R1; TRB7 |
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| OMIM = 604796 |
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| ECnumber = |
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| Homologene = 10480 |
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| MGIid = 2681253 |
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| GeneAtlas_image1 = PBB_GE_TAS2R1_221324_at_tn.png |
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| Function = {{GNF_GO|id=GO:0004872 |text = receptor activity}} {{GNF_GO|id=GO:0004930 |text = G-protein coupled receptor activity}} {{GNF_GO|id=GO:0008527 |text = taste receptor activity}} |
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| Component = {{GNF_GO|id=GO:0016020 |text = membrane}} {{GNF_GO|id=GO:0016021 |text = integral to membrane}} |
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| Process = {{GNF_GO|id=GO:0007165 |text = signal transduction}} {{GNF_GO|id=GO:0007186 |text = G-protein coupled receptor protein signaling pathway}} {{GNF_GO|id=GO:0007635 |text = chemosensory behavior}} {{GNF_GO|id=GO:0050896 |text = response to stimulus}} {{GNF_GO|id=GO:0050909 |text = sensory perception of taste}} |
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| Orthologs = {{GNF_Ortholog_box |
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| Hs_EntrezGene = 50834 |
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| Hs_Ensembl = ENSG00000169777 |
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| Hs_RefseqProtein = NP_062545 |
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| Hs_RefseqmRNA = NM_019599 |
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| Hs_GenLoc_db = |
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| Hs_GenLoc_chr = 5 |
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| Hs_GenLoc_start = 9682115 |
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| Hs_GenLoc_end = 9683467 |
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| Hs_Uniprot = Q9NYW7 |
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| Mm_EntrezGene = 57254 |
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| Mm_Ensembl = ENSMUSG00000045267 |
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| Mm_RefseqmRNA = NM_020503 |
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| Mm_RefseqProtein = NP_065249 |
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| Mm_GenLoc_db = |
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| Mm_GenLoc_chr = 15 |
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| Mm_GenLoc_start = 32121876 |
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| Mm_GenLoc_end = 32122883 |
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| Mm_Uniprot = Q9JKT2 |
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}} |
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}} |
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'''Taste receptor, type 2, member 1''', also known as '''TAS2R1''', is a human [[gene]].<ref name="entrez">{{cite web | title = Entrez Gene: TAS2R1 taste receptor, type 2, member 1| url = http://www.ncbi.nlm.nih.gov/sites/entrez?Db=gene&Cmd=ShowDetailView&TermToSearch=50834| accessdate = }}</ref> |
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=== Extra-oral roles of TAS2Rs === |
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Bitter taste receptors are expressed in [[Taste receptor|taste receptor cells]], which organized into [[taste bud]]s on the papillae of the [[tongue]] and palate epithelium. |
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{{PBB_Summary |
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| section_title = |
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In addition, TAS2Rs were found to be expressed in extra-oral tissues, e.g. brain, lungs, gastrointestinal tract, etc.<ref name=":1" /> So far, less is known about their function however, for example it was shown that: |
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| summary_text = This gene encodes a member of a family of candidate taste receptors that are members of the G protein-coupled receptor superfamily and that are specifically expressed by taste receptor cells of the tongue and palate epithelia. This intronless taste receptor gene encodes a 7-transmembrane receptor protein, functioning as a bitter taste receptor. This gene is mapped to chromosome 5p15, the location of a genetic locus (PROP) that controls the detection of the bitter compound 6-n-propyl-2-thiouracil.<ref name="entrez">{{cite web | title = Entrez Gene: TAS2R1 taste receptor, type 2, member 1| url = http://www.ncbi.nlm.nih.gov/sites/entrez?Db=gene&Cmd=ShowDetailView&TermToSearch=50834| accessdate = }}</ref> |
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}} |
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* TAS2Rs mediate relaxation of airway smooth muscles.<ref>{{Cite journal|last1=Deshpande|first1=Deepak A|last2=Wang|first2=Wayne C H|last3=McIlmoyle|first3=Elizabeth L|last4=Robinett|first4=Kathryn S|last5=Schillinger|first5=Rachel M|last6=An|first6=Steven S|last7=Sham|first7=James S K|last8=Liggett|first8=Stephen B|date=2010-10-24|title=Bitter taste receptors on airway smooth muscle bronchodilate by localized calcium signaling and reverse obstruction|journal=Nature Medicine|volume=16|issue=11|pages=1299–1304|doi=10.1038/nm.2237|pmid=20972434|issn=1078-8956|pmc=3066567}}</ref> |
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* TAS2R43 is involved in secretion of gastric acid in the stomach.<ref>{{Cite journal|last1=Liszt|first1=Kathrin Ingrid|last2=Ley|first2=Jakob Peter|last3=Lieder|first3=Barbara|last4=Behrens|first4=Maik|last5=Stöger|first5=Verena|last6=Reiner|first6=Angelika|last7=Hochkogler|first7=Christina Maria|last8=Köck|first8=Elke|last9=Marchiori|first9=Alessandro|date=2017-07-10|title=Caffeine induces gastric acid secretion via bitter taste signaling in gastric parietal cells|journal=Proceedings of the National Academy of Sciences|volume=114|issue=30|pages=E6260–E6269|doi=10.1073/pnas.1703728114|pmid=28696284|issn=0027-8424|pmc=5544304|doi-access=free|bibcode=2017PNAS..114E6260L }}</ref> |
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=== Extra-oral roles of TAS2R1 === |
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* TAS2R1, TAS2R4, TAS2R10, TAS2R38 and TAS2R49 were found to be down-regulated in [[breast cancer]] cells<ref>{{Cite journal|last1=Singh|first1=Nisha|last2=Chakraborty|first2=Raja|last3=Bhullar|first3=Rajinder Pal|last4=Chelikani|first4=Prashen|date=April 2014|title=Differential expression of bitter taste receptors in non-cancerous breast epithelial and breast cancer cells|journal=Biochemical and Biophysical Research Communications|volume=446|issue=2|pages=499–503|doi=10.1016/j.bbrc.2014.02.140|pmid=24613843|issn=0006-291X}}</ref>''.'' |
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* TAS2R1, causes vasoconstrictor responses in the [[Pulmonary circulation|pulmonary circuit]] and relaxation in the airways<ref>{{Cite journal|last1=Upadhyaya|first1=Jasbir D.|last2=Singh|first2=Nisha|last3=Sikarwar|first3=Anurag S.|last4=Chakraborty|first4=Raja|last5=Pydi|first5=Sai P.|last6=Bhullar|first6=Rajinder P.|last7=Dakshinamurti|first7=Shyamala|last8=Chelikani|first8=Prashen|date=2014-10-23|title=Dextromethorphan Mediated Bitter Taste Receptor Activation in the Pulmonary Circuit Causes Vasoconstriction|journal=PLOS ONE|volume=9|issue=10|pages=e110373|doi=10.1371/journal.pone.0110373|pmid=25340739|pmc=4207743|issn=1932-6203|bibcode=2014PLoSO...9k0373U|doi-access=free}}</ref>''.'' |
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== Structure of TAS2R1 receptor == |
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Based on a recent [[Homology modeling|homology model]] from [http://bitterdb.agri.huji.ac.il/Receptor.php?mode_organism=default&id=1 BitterDB]<ref>{{Cite journal|last1=Wiener|first1=Ayana|last2=Shudler|first2=Marina|last3=Levit|first3=Anat|last4=Niv|first4=Masha Y.|date=2011-09-22|title=BitterDB: a database of bitter compounds|journal=Nucleic Acids Research|volume=40|issue=D1|pages=D413–D419|doi=10.1093/nar/gkr755|pmid=21940398|pmc=3245057|issn=1362-4962}}</ref><ref>{{Cite journal|last1=Dagan-Wiener|first1=Ayana|last2=Di Pizio|first2=Antonella|last3=Nissim|first3=Ido|last4=Bahia|first4=Malkeet S|last5=Dubovski|first5=Nitzan|last6=Margulis|first6=Eitan|last7=Niv|first7=Masha Y|date=2018-10-24|title=BitterDB: taste ligands and receptors database in 2019|journal=Nucleic Acids Research|volume=47|issue=D1|pages=D1179–D1185|doi=10.1093/nar/gky974|pmid=30357384|pmc=6323989|issn=0305-1048}}</ref> several conserved [[Structural motif|motifs]], which are counterparts to Class A GPCRs<ref name=":0" /> were found: |
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* Transmembrane helix 1: N<sup>1.50</sup>xxI<sup>1.53</sup> |
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* Transmembrane helix 2: L<sup>2.46</sup>xxxR<sup>2.50</sup> |
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* Transmembrane helix 3: F<sup>3.49</sup>Y<sup>3.50</sup>xxK<sup>3.53</sup> |
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* Transmembrane helix 5: P<sup>5.50</sup> |
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* Transmembrane helix 6: F<sup>6.44</sup>xxxY<sup>6.46</sup> |
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* Transmembrane helix 7: H<sup>7.49</sup>S<sup>7.50</sup>xxL<sup>7.53</sup> |
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<small>Numbering is according to the Balleros-Weinstein</small><ref>{{Citation|last1=Ballesteros|first1=Juan A.|title=[19] Integrated methods for the construction of three-dimensional models and computational probing of structure-function relations in G protein-coupled receptors|date=1995|work=Methods in Neurosciences|pages=366–428|publisher=Elsevier|isbn=978-0-12-185295-5|last2=Weinstein|first2=Harel|doi=10.1016/s1043-9471(05)80049-7}}</ref> <small>system.</small> |
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Unlike in Class A GPCRs, in transmembrane helix 4 no DRY<ref>{{Cite journal|last1=Rovati|first1=G. E.|last2=Capra|first2=V.|last3=Neubig|first3=R. R.|date=2007-01-12|title=The Highly Conserved DRY Motif of Class A G Protein-Coupled Receptors: Beyond the Ground State|journal=Molecular Pharmacology|volume=71|issue=4|pages=959–964|doi=10.1124/mol.106.029470|pmid=17192495|s2cid=15536186|issn=0026-895X}}</ref> motif was found as well as position 6.50 is not conserved. |
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== ''TAS2R1'' gene == |
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This gene encodes a member of a family of candidate [[taste receptor]]s that are members of the [[G protein-coupled receptor]] superfamily and that are specifically expressed by taste receptor cells of the tongue and palate epithelia. This intronless taste receptor gene encodes a 7-transmembrane receptor protein, functioning as a bitter taste receptor. |
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=== SNPs === |
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In T2R1 two [[Single-nucleotide polymorphism|SNPs]] are known in R111H and R206W ([[dbSNP]]). |
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=== Transcription factors === |
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So far, AML1a, AP-1, AREB6, FOXL1, IRF-7A, Lmo2, NF-E2, NF-E2 p45 were found as the top [[transcription factor]] binding sites by QIAGEN in the [https://www.genecards.org/cgi-bin/carddisp.pl?gene=TAS2R1 ''TAS2R1'' gene promoter]. |
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=== [[Site-directed mutagenesis|Mutagenesis]] data === |
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Several [[mutation]]s have been shown to influence binding of a ligand to TAS2R1 (based on [http://bitterdb.agri.huji.ac.il/Receptor.php?mode_organism=default&id=1 BitterDB]): |
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{| class="wikitable" |
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|Receptor region |
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|BW number |
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|Residue |
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|Reference |
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|- |
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|TM1 |
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|1.5 |
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|N24 |
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|doi: 10.1021/acs.jctc.5b00472 doi: 10.1074/jbc.M111.246983 |
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|- |
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|TM1 |
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|1.53 |
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|I27 |
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|doi: 10.1021/acs.jctc.5b00472 doi: 10.1074/jbc.M111.246983 |
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|- |
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|TM2 |
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|2.5 |
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|R55 |
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|doi: 10.1021/acs.jctc.5b00472 doi: 10.1074/jbc.M111.246983 |
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|- |
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|TM2 |
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|2.56 |
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|F61 |
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|doi: 10.1074/jbc.M111.246983 |
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|- |
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|TM2 |
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|2.61 |
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|N66 |
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|doi: 10.3389/fmolb.2017.00063 doi: 10.1021/acs.jctc.5b00472 doi: 10.1016/bs.mcb.2015.10.005 doi: 10.1074/jbc.M111.246983 |
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|- |
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|ECL1 |
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| |
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|E74 |
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|doi: 10.3389/fmolb.2017.00063 |
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|- |
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|TM3 |
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|3.32 |
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|L85 |
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|doi: 10.1016/bs.mcb.2015.10.005 doi: 10.3109/10799893.2011.578141 |
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|- |
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|TM3 |
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|3.33 |
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|L86 |
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|doi: 10.1016/bs.mcb.2015.10.005 doi: 10.3109/10799893.2011.578141 |
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|- |
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|TM3 |
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|3.36 |
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|N89 |
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|doi: 10.1016/bs.mcb.2015.10.005 doi: 10.1074/jbc.M111.246983 doi: doi: 10.1021/acs.jctc.5b00472 doi: 10.1074/jbc.M111.246983 |
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|- |
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|TM3 |
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|3.37 |
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|E90 |
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|doi: 10.1016/bs.mcb.2015.10.005 doi: 10.3109/10799893.2011.578141 |
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|- |
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|TM3 |
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|3.41 |
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|W94 |
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|doi: 10.1021/acs.jctc.5b00472 doi: 10.1074/jbc.M111.246983 |
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|- |
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|TM3 |
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|3.46 |
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|L99 |
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|doi: 10.1021/acs.jctc.5b00472 doi: 10.1074/jbc.M111.246983 |
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|- |
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|TM5 |
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|5.46 |
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|E182 |
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|doi: 10.1016/bs.mcb.2015.10.005 doi: 10.3109/10799893.2011.578141 |
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|- |
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|TM5 |
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|5.61 |
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|L197 |
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|doi: 10.1021/acs.jctc.5b00472 doi: 10.1074/jbc.M111.246983 |
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|- |
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|TM5 |
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|5.64 |
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|S200 |
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|doi: 10.1021/acs.jctc.5b00472 doi: 10.1074/jbc.M111.246983 |
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|- |
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|TM5 |
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|5.65 |
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|L201 |
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|doi: 10.1021/acs.jctc.5b00472 doi: 10.1074/jbc.M111.246983 |
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|- |
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|TM7 |
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|7.39 |
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|I263 |
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|doi: 10.1016/bs.mcb.2015.10.005 doi: 10.3109/10799893.2011.578141 |
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|- |
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|TM7 |
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|7.49 |
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|H273 |
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|doi: 10.1021/acs.jctc.5b00472 doi: 10.1074/jbc.M111.246983 |
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|- |
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|TM7 |
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|7.53 |
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|L277 |
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|doi: 10.1021/acs.jctc.5b00472 doi: 10.1074/jbc.M111.246983 |
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|- |
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|TM7 |
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|7.54 |
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|I278 |
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|doi: 10.1021/acs.jctc.5b00472 doi: 10.1074/jbc.M111.246983 |
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|} |
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== Ligands == |
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Up to now, 39 ligands for T2R1 were identified in [[BitterDB]], among them [[Amino acid|L-amino acids]], [[peptide]]s, [[humulone]]s, small molecules etc.<ref>{{BitterDB receptor|id=1|title=hTAS2R1}}</ref> |
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==See also== |
==See also== |
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==Further reading== |
==Further reading== |
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{{refbegin | 2}} |
{{refbegin | 2}} |
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*{{cite journal | author = Kinnamon SC | title = A plethora of taste receptors | journal = Neuron | volume = 25 | issue = 3 | pages = 507–10 | year = 2000 | pmid = 10774719 | doi = 10.1016/S0896-6273(00)81054-5 | doi-access = free }} |
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{{PBB_Further_reading |
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*{{cite journal | author = Margolskee RF | title = Molecular mechanisms of bitter and sweet taste transduction | journal = J. Biol. Chem. | volume = 277 | issue = 1 | pages = 1–4 | year = 2002 | pmid = 11696554 | doi = 10.1074/jbc.R100054200 | doi-access = free }} |
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| citations = |
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*{{cite journal |
*{{cite journal | vauthors = Montmayeur JP, Matsunami H | title = Receptors for bitter and sweet taste | journal = Curr. Opin. Neurobiol. | volume = 12 | issue = 4 | pages = 366–71 | year = 2002 | pmid = 12139982 | doi = 10.1016/S0959-4388(02)00345-8 | s2cid = 37807140 }} |
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*{{cite journal |
*{{cite journal | vauthors = Chandrashekar J, Mueller KL, Hoon MA, Adler E, Feng L, Guo W, Zuker CS, Ryba NJ | title = T2Rs function as bitter taste receptors | journal = Cell | volume = 100 | issue = 6 | pages = 703–11 | year = 2000 | pmid = 10761935 | doi = 10.1016/S0092-8674(00)80706-0 | s2cid = 7293493 | doi-access = free }} |
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*{{cite journal |
*{{cite journal | author = Firestein S | title = The good taste of genomics | journal = Nature | volume = 404 | issue = 6778 | pages = 552–3 | year = 2000 | pmid = 10766221 | doi = 10.1038/35007167 | s2cid = 35741332 | doi-access = free }} |
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*{{cite journal |
*{{cite journal | vauthors = Zhang Y, Hoon MA, Chandrashekar J, Mueller KL, Cook B, Wu D, Zuker CS, Ryba NJ | title = Coding of sweet, bitter, and umami tastes: different receptor cells sharing similar signaling pathways | journal = Cell | volume = 112 | issue = 3 | pages = 293–301 | year = 2003 | pmid = 12581520 | doi = 10.1016/S0092-8674(03)00071-0 | s2cid = 718601 | doi-access = free }} |
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*{{cite journal |
*{{cite journal | vauthors = Fischer A, Gilad Y, Man O, Pääbo S | title = Evolution of bitter taste receptors in humans and apes | journal = Mol. Biol. Evol. | volume = 22 | issue = 3 | pages = 432–6 | year = 2005 | pmid = 15496549 | doi = 10.1093/molbev/msi027 | doi-access = free }} |
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*{{cite journal |
*{{cite journal | vauthors = Go Y, Satta Y, Takenaka O, Takahata N | title = Lineage-specific loss of function of bitter taste receptor genes in humans and nonhuman primates | journal = Genetics | volume = 170 | issue = 1 | pages = 313–26 | year = 2006 | pmid = 15744053 | pmc = 1449719 | doi = 10.1534/genetics.104.037523 }} |
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*{{cite journal | author=Matsunami H, Montmayeur JP, Buck LB |title=A family of candidate taste receptors in human and mouse. |journal=Nature |volume=404 |issue= 6778 |pages= 601-4 |year= 2000 |pmid= 10766242 |doi= 10.1038/35007072 }} |
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*{{cite journal | author=Strausberg RL, Feingold EA, Grouse LH, ''et al.'' |title=Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences. |journal=Proc. Natl. Acad. Sci. U.S.A. |volume=99 |issue= 26 |pages= 16899-903 |year= 2003 |pmid= 12477932 |doi= 10.1073/pnas.242603899 }} |
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*{{cite journal | author=Zhang Y, Hoon MA, Chandrashekar J, ''et al.'' |title=Coding of sweet, bitter, and umami tastes: different receptor cells sharing similar signaling pathways. |journal=Cell |volume=112 |issue= 3 |pages= 293-301 |year= 2003 |pmid= 12581520 |doi= }} |
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*{{cite journal | author=Gerhard DS, Wagner L, Feingold EA, ''et al.'' |title=The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC). |journal=Genome Res. |volume=14 |issue= 10B |pages= 2121-7 |year= 2004 |pmid= 15489334 |doi= 10.1101/gr.2596504 }} |
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*{{cite journal | author=Fischer A, Gilad Y, Man O, Pääbo S |title=Evolution of bitter taste receptors in humans and apes. |journal=Mol. Biol. Evol. |volume=22 |issue= 3 |pages= 432-6 |year= 2005 |pmid= 15496549 |doi= 10.1093/molbev/msi027 }} |
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*{{cite journal | author=Go Y, Satta Y, Takenaka O, Takahata N |title=Lineage-specific loss of function of bitter taste receptor genes in humans and nonhuman primates. |journal=Genetics |volume=170 |issue= 1 |pages= 313-26 |year= 2006 |pmid= 15744053 |doi= 10.1534/genetics.104.037523 }} |
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}} |
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{{refend}} |
{{refend}} |
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{{ |
{{G protein-coupled receptors|g3}} |
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{{NLM content}} |
{{NLM content}} |
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{{G protein-coupled receptors}} |
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[[Category: |
[[Category:Human taste receptors]] |
Latest revision as of 23:52, 9 March 2024
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Aliases | TAS2R1, T2R1, TRB7, taste 2 receptor member 1 | ||||||||||||||||||||||||||||||||||||||||||||||||||
External IDs | OMIM: 604796; MGI: 2681253; HomoloGene: 10480; GeneCards: TAS2R1; OMA:TAS2R1 - orthologs | ||||||||||||||||||||||||||||||||||||||||||||||||||
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Taste receptor type 2 member 1 (TAS2R1/T2R1) is a protein that in humans is encoded by the TAS2R1 gene.[5][6][7] It belongs to the G protein-coupled receptor (GPCR) family and is related to class A-like GPCRs, they contain 7 transmembrane helix bundles and short N-terminus loop.[8] Furthermore, TAS2R1 is member of the 25 known human bitter taste receptors, which enable the perception of bitter taste in the mouth cavity. Increasing evidence indicates a functional role of TAS2Rs in extra-oral tissues.[9]
Expression and function
[edit]Extra-oral roles of TAS2Rs
[edit]Bitter taste receptors are expressed in taste receptor cells, which organized into taste buds on the papillae of the tongue and palate epithelium.
In addition, TAS2Rs were found to be expressed in extra-oral tissues, e.g. brain, lungs, gastrointestinal tract, etc.[9] So far, less is known about their function however, for example it was shown that:
- TAS2Rs mediate relaxation of airway smooth muscles.[10]
- TAS2R43 is involved in secretion of gastric acid in the stomach.[11]
Extra-oral roles of TAS2R1
[edit]- TAS2R1, TAS2R4, TAS2R10, TAS2R38 and TAS2R49 were found to be down-regulated in breast cancer cells[12].
- TAS2R1, causes vasoconstrictor responses in the pulmonary circuit and relaxation in the airways[13].
Structure of TAS2R1 receptor
[edit]Based on a recent homology model from BitterDB[14][15] several conserved motifs, which are counterparts to Class A GPCRs[8] were found:
- Transmembrane helix 1: N1.50xxI1.53
- Transmembrane helix 2: L2.46xxxR2.50
- Transmembrane helix 3: F3.49Y3.50xxK3.53
- Transmembrane helix 5: P5.50
- Transmembrane helix 6: F6.44xxxY6.46
- Transmembrane helix 7: H7.49S7.50xxL7.53
Numbering is according to the Balleros-Weinstein[16] system.
Unlike in Class A GPCRs, in transmembrane helix 4 no DRY[17] motif was found as well as position 6.50 is not conserved.
TAS2R1 gene
[edit]This gene encodes a member of a family of candidate taste receptors that are members of the G protein-coupled receptor superfamily and that are specifically expressed by taste receptor cells of the tongue and palate epithelia. This intronless taste receptor gene encodes a 7-transmembrane receptor protein, functioning as a bitter taste receptor.
SNPs
[edit]In T2R1 two SNPs are known in R111H and R206W (dbSNP).
Transcription factors
[edit]So far, AML1a, AP-1, AREB6, FOXL1, IRF-7A, Lmo2, NF-E2, NF-E2 p45 were found as the top transcription factor binding sites by QIAGEN in the TAS2R1 gene promoter.
Mutagenesis data
[edit]Several mutations have been shown to influence binding of a ligand to TAS2R1 (based on BitterDB):
Receptor region | BW number | Residue | Reference |
TM1 | 1.5 | N24 | doi: 10.1021/acs.jctc.5b00472 doi: 10.1074/jbc.M111.246983 |
TM1 | 1.53 | I27 | doi: 10.1021/acs.jctc.5b00472 doi: 10.1074/jbc.M111.246983 |
TM2 | 2.5 | R55 | doi: 10.1021/acs.jctc.5b00472 doi: 10.1074/jbc.M111.246983 |
TM2 | 2.56 | F61 | doi: 10.1074/jbc.M111.246983 |
TM2 | 2.61 | N66 | doi: 10.3389/fmolb.2017.00063 doi: 10.1021/acs.jctc.5b00472 doi: 10.1016/bs.mcb.2015.10.005 doi: 10.1074/jbc.M111.246983 |
ECL1 | E74 | doi: 10.3389/fmolb.2017.00063 | |
TM3 | 3.32 | L85 | doi: 10.1016/bs.mcb.2015.10.005 doi: 10.3109/10799893.2011.578141 |
TM3 | 3.33 | L86 | doi: 10.1016/bs.mcb.2015.10.005 doi: 10.3109/10799893.2011.578141 |
TM3 | 3.36 | N89 | doi: 10.1016/bs.mcb.2015.10.005 doi: 10.1074/jbc.M111.246983 doi: doi: 10.1021/acs.jctc.5b00472 doi: 10.1074/jbc.M111.246983 |
TM3 | 3.37 | E90 | doi: 10.1016/bs.mcb.2015.10.005 doi: 10.3109/10799893.2011.578141 |
TM3 | 3.41 | W94 | doi: 10.1021/acs.jctc.5b00472 doi: 10.1074/jbc.M111.246983 |
TM3 | 3.46 | L99 | doi: 10.1021/acs.jctc.5b00472 doi: 10.1074/jbc.M111.246983 |
TM5 | 5.46 | E182 | doi: 10.1016/bs.mcb.2015.10.005 doi: 10.3109/10799893.2011.578141 |
TM5 | 5.61 | L197 | doi: 10.1021/acs.jctc.5b00472 doi: 10.1074/jbc.M111.246983 |
TM5 | 5.64 | S200 | doi: 10.1021/acs.jctc.5b00472 doi: 10.1074/jbc.M111.246983 |
TM5 | 5.65 | L201 | doi: 10.1021/acs.jctc.5b00472 doi: 10.1074/jbc.M111.246983 |
TM7 | 7.39 | I263 | doi: 10.1016/bs.mcb.2015.10.005 doi: 10.3109/10799893.2011.578141 |
TM7 | 7.49 | H273 | doi: 10.1021/acs.jctc.5b00472 doi: 10.1074/jbc.M111.246983 |
TM7 | 7.53 | L277 | doi: 10.1021/acs.jctc.5b00472 doi: 10.1074/jbc.M111.246983 |
TM7 | 7.54 | I278 | doi: 10.1021/acs.jctc.5b00472 doi: 10.1074/jbc.M111.246983 |
Ligands
[edit]Up to now, 39 ligands for T2R1 were identified in BitterDB, among them L-amino acids, peptides, humulones, small molecules etc.[18]
See also
[edit]References
[edit]- ^ a b c GRCh38: Ensembl release 89: ENSG00000169777 – Ensembl, May 2017
- ^ a b c GRCm38: Ensembl release 89: ENSMUSG00000045267 – Ensembl, May 2017
- ^ "Human PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
- ^ "Mouse PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
- ^ Adler E, Hoon MA, Mueller KL, Chandrashekar J, Ryba NJ, Zuker CS (Apr 2000). "A novel family of mammalian taste receptors". Cell. 100 (6): 693–702. doi:10.1016/S0092-8674(00)80705-9. PMID 10761934. S2CID 14604586.
- ^ Matsunami H, Montmayeur JP, Buck LB (Apr 2000). "A family of candidate taste receptors in human and mouse". Nature. 404 (6778): 601–4. Bibcode:2000Natur.404..601M. doi:10.1038/35007072. PMID 10766242. S2CID 4336913.
- ^ "Entrez Gene: TAS2R1 taste receptor, type 2, member 1".
- ^ a b Di Pizio A, Levit A, Slutzki M, Behrens M, Karaman R, Niv MY (2016), "Comparing Class a GPCRS to bitter taste receptors", G Protein-Coupled Receptors - Signaling, Trafficking and Regulation, Methods in Cell Biology, vol. 132, Elsevier, pp. 401–427, doi:10.1016/bs.mcb.2015.10.005, ISBN 978-0-12-803595-5, PMID 26928553
- ^ a b Lu P, Zhang CH, Lifshitz LM, ZhuGe R (2017-01-04). "Extraoral bitter taste receptors in health and disease". The Journal of General Physiology. 149 (2): 181–197. doi:10.1085/jgp.201611637. ISSN 0022-1295. PMC 5299619. PMID 28053191.
- ^ Deshpande DA, Wang WC, McIlmoyle EL, Robinett KS, Schillinger RM, An SS, Sham JS, Liggett SB (2010-10-24). "Bitter taste receptors on airway smooth muscle bronchodilate by localized calcium signaling and reverse obstruction". Nature Medicine. 16 (11): 1299–1304. doi:10.1038/nm.2237. ISSN 1078-8956. PMC 3066567. PMID 20972434.
- ^ Liszt KI, Ley JP, Lieder B, Behrens M, Stöger V, Reiner A, Hochkogler CM, Köck E, Marchiori A (2017-07-10). "Caffeine induces gastric acid secretion via bitter taste signaling in gastric parietal cells". Proceedings of the National Academy of Sciences. 114 (30): E6260–E6269. Bibcode:2017PNAS..114E6260L. doi:10.1073/pnas.1703728114. ISSN 0027-8424. PMC 5544304. PMID 28696284.
- ^ Singh N, Chakraborty R, Bhullar RP, Chelikani P (April 2014). "Differential expression of bitter taste receptors in non-cancerous breast epithelial and breast cancer cells". Biochemical and Biophysical Research Communications. 446 (2): 499–503. doi:10.1016/j.bbrc.2014.02.140. ISSN 0006-291X. PMID 24613843.
- ^ Upadhyaya JD, Singh N, Sikarwar AS, Chakraborty R, Pydi SP, Bhullar RP, Dakshinamurti S, Chelikani P (2014-10-23). "Dextromethorphan Mediated Bitter Taste Receptor Activation in the Pulmonary Circuit Causes Vasoconstriction". PLOS ONE. 9 (10): e110373. Bibcode:2014PLoSO...9k0373U. doi:10.1371/journal.pone.0110373. ISSN 1932-6203. PMC 4207743. PMID 25340739.
- ^ Wiener A, Shudler M, Levit A, Niv MY (2011-09-22). "BitterDB: a database of bitter compounds". Nucleic Acids Research. 40 (D1): D413–D419. doi:10.1093/nar/gkr755. ISSN 1362-4962. PMC 3245057. PMID 21940398.
- ^ Dagan-Wiener A, Di Pizio A, Nissim I, Bahia MS, Dubovski N, Margulis E, Niv MY (2018-10-24). "BitterDB: taste ligands and receptors database in 2019". Nucleic Acids Research. 47 (D1): D1179–D1185. doi:10.1093/nar/gky974. ISSN 0305-1048. PMC 6323989. PMID 30357384.
- ^ Ballesteros JA, Weinstein H (1995), "[19] Integrated methods for the construction of three-dimensional models and computational probing of structure-function relations in G protein-coupled receptors", Methods in Neurosciences, Elsevier, pp. 366–428, doi:10.1016/s1043-9471(05)80049-7, ISBN 978-0-12-185295-5
- ^ Rovati GE, Capra V, Neubig RR (2007-01-12). "The Highly Conserved DRY Motif of Class A G Protein-Coupled Receptors: Beyond the Ground State". Molecular Pharmacology. 71 (4): 959–964. doi:10.1124/mol.106.029470. ISSN 0026-895X. PMID 17192495. S2CID 15536186.
- ^ "hTAS2R1". BitterDB. The Hebrew University of Jerusalem.
Further reading
[edit]- Kinnamon SC (2000). "A plethora of taste receptors". Neuron. 25 (3): 507–10. doi:10.1016/S0896-6273(00)81054-5. PMID 10774719.
- Margolskee RF (2002). "Molecular mechanisms of bitter and sweet taste transduction". J. Biol. Chem. 277 (1): 1–4. doi:10.1074/jbc.R100054200. PMID 11696554.
- Montmayeur JP, Matsunami H (2002). "Receptors for bitter and sweet taste". Curr. Opin. Neurobiol. 12 (4): 366–71. doi:10.1016/S0959-4388(02)00345-8. PMID 12139982. S2CID 37807140.
- Chandrashekar J, Mueller KL, Hoon MA, Adler E, Feng L, Guo W, Zuker CS, Ryba NJ (2000). "T2Rs function as bitter taste receptors". Cell. 100 (6): 703–11. doi:10.1016/S0092-8674(00)80706-0. PMID 10761935. S2CID 7293493.
- Firestein S (2000). "The good taste of genomics". Nature. 404 (6778): 552–3. doi:10.1038/35007167. PMID 10766221. S2CID 35741332.
- Zhang Y, Hoon MA, Chandrashekar J, Mueller KL, Cook B, Wu D, Zuker CS, Ryba NJ (2003). "Coding of sweet, bitter, and umami tastes: different receptor cells sharing similar signaling pathways". Cell. 112 (3): 293–301. doi:10.1016/S0092-8674(03)00071-0. PMID 12581520. S2CID 718601.
- Fischer A, Gilad Y, Man O, Pääbo S (2005). "Evolution of bitter taste receptors in humans and apes". Mol. Biol. Evol. 22 (3): 432–6. doi:10.1093/molbev/msi027. PMID 15496549.
- Go Y, Satta Y, Takenaka O, Takahata N (2006). "Lineage-specific loss of function of bitter taste receptor genes in humans and nonhuman primates". Genetics. 170 (1): 313–26. doi:10.1534/genetics.104.037523. PMC 1449719. PMID 15744053.
This article incorporates text from the United States National Library of Medicine, which is in the public domain.