Pseudo-response regulator: Difference between revisions
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== Discovery == |
== Discovery == |
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Multiple labs identified the PRR genes as parts of the circadian clock in the 1990s. In 2000, Akinori Matsushika, Seiya Makino, Masaya Kojima, and Takeshi Mizuno were the first to understand PRR genes as pseudo-response repressor genes rather than as [[Response regulator|response regulator (ARR) genes]].<ref name=":4">{{Cite journal|last=Salomé|first=Patrice A.|last2=McClung|first2=C. Robertson|title=The Arabidopsis thaliana Clock|url=http://journals.sagepub.com.libproxy.wustl.edu/doi/pdf/10.1177/0748730404268112|journal=Journal of Biological Rhythms|volume=19|issue=5|pages=425–435|doi=10.1177/0748730404268112}}</ref><ref>{{Cite journal|last=Boss|first=Paul K.|last2=Bastow|first2=Ruth M.|last3=Mylne|first3=Joshua S.|last4=Dean|first4=Caroline|date=2004-06-01|title=Multiple Pathways in the Decision to Flower: Enabling, Promoting, and Resetting|url=http://www.plantcell.org/content/16/suppl_1/S18|journal=The Plant Cell|language=en|volume=16|issue=suppl 1|pages=S18–S31|doi=10.1105/tpc.015958|issn=1532-298X|pmc=2643402|pmid=15037730}}</ref> The factor that distinguishes PRR from ARR genes is the lack of a phospho-accepting [[aspartate]] site that characterizes ARR proteins. Though their research that discovered PRR genes was primarily hailed during the early 2000s as informing the scientific community about the function of [[TOC1 (gene)|TOC1]], an additional pseudo-response regulator in the ''[[Arabidopsis thaliana]]'' biological clock,<ref>{{Cite journal|last=Alabadı́|first=David|last2=Oyama|first2=Tokitaka|last3=Yanovsky|first3=Marcelo J.|last4=Harmon|first4=Franklin G.|last5=Más|first5=Paloma|last6=Kay|first6=Steve A.|date=2001-08-03|title=Reciprocal Regulation Between TOC1 and LHY/CCA1 Within the Arabidopsis Circadian Clock|url=http://science.sciencemag.org/content/293/5531/880|journal=Science|language=en|volume=293|issue=5531|pages=880–883|doi=10.1126/science.1061320|issn=0036-8075|pmid=11486091}}</ref> the information about PRR genes that Matsushika and his team found deepened scientific understanding of circadian clocks in plants and led other researchers to hypothesize about the purpose of the PRR genes<ref name=":4" /> |
Multiple labs identified the PRR genes as parts of the circadian clock in the 1990s. In 2000, Akinori Matsushika, Seiya Makino, Masaya Kojima, and Takeshi Mizuno were the first to understand PRR genes as pseudo-response repressor genes rather than as [[Response regulator|response regulator (ARR) genes]].<ref name=":4">{{Cite journal|last=Salomé|first=Patrice A.|last2=McClung|first2=C. Robertson|title=The Arabidopsis thaliana Clock|url=http://journals.sagepub.com.libproxy.wustl.edu/doi/pdf/10.1177/0748730404268112|journal=Journal of Biological Rhythms|volume=19|issue=5|pages=425–435|doi=10.1177/0748730404268112}}</ref><ref>{{Cite journal|last=Boss|first=Paul K.|last2=Bastow|first2=Ruth M.|last3=Mylne|first3=Joshua S.|last4=Dean|first4=Caroline|date=2004-06-01|title=Multiple Pathways in the Decision to Flower: Enabling, Promoting, and Resetting|url=http://www.plantcell.org/content/16/suppl_1/S18|journal=The Plant Cell|language=en|volume=16|issue=suppl 1|pages=S18–S31|doi=10.1105/tpc.015958|issn=1532-298X|pmc=2643402|pmid=15037730}}</ref> The factor that distinguishes PRR from ARR genes is the lack of a phospho-accepting [[aspartate]] site that characterizes ARR proteins. Though their research that discovered PRR genes was primarily hailed during the early 2000s as informing the scientific community about the function of [[TOC1 (gene)|TOC1]], an additional pseudo-response regulator in the ''[[Arabidopsis thaliana]]'' biological clock,<ref>{{Cite journal|last=Alabadı́|first=David|last2=Oyama|first2=Tokitaka|last3=Yanovsky|first3=Marcelo J.|last4=Harmon|first4=Franklin G.|last5=Más|first5=Paloma|last6=Kay|first6=Steve A.|date=2001-08-03|title=Reciprocal Regulation Between TOC1 and LHY/CCA1 Within the Arabidopsis Circadian Clock|url=http://science.sciencemag.org/content/293/5531/880|journal=Science|language=en|volume=293|issue=5531|pages=880–883|doi=10.1126/science.1061320|issn=0036-8075|pmid=11486091}}</ref> the information about PRR genes that Matsushika and his team found deepened scientific understanding of circadian clocks in plants and led other researchers to hypothesize about the purpose of the PRR genes.<ref name=":4" /> Though current research has identified PRR3, PRR5, PRR7, and PRR9 as of importance to the ''A. thaliana'' circadian clock mechanism, Matsushika et al. first categorized PRR genes into two subgroups (APRR1 and APRR2, the A stands for Arabidopsis) due to two differing amino acid structures.<ref name=":0">{{Cite journal|last=Matsushika|first=Akinori|date=2000|title=Circadian Waves of Expression of the APRR1/TOC1 Family of Pseudo-Response Regulators in Arabidopsis thaliana: Insight into the Plant Circadian Clock|url=https://academic.oup.com/pcp/article/41/9/1002/2329572/Circadian-Waves-of-Expression-of-the-APRR1-TOC1|journal=Plant Cell Physiology|volume=41|pages=1002–1012}}</ref> The idea that the PRR genes formed parts of a complex [[repressilator]] of [[Negative feedback|negative feedback loops]] was conceived by [[Andrew Millar (scientist)|Andrew Millar]]’s lab in 2012.<ref>{{Cite journal|last=Pokhilko|first=Alexandra|last2=Fernández|first2=Aurora Piñas|last3=Edwards|first3=Kieron D.|last4=Southern|first4=Megan M.|last5=Halliday|first5=Karen J.|last6=Millar|first6=Andrew J.|date=2012-01-01|title=The clock gene circuit in Arabidopsis includes a repressilator with additional feedback loops|url=http://msb.embopress.org/content/8/1/574|journal=Molecular Systems Biology|language=en|volume=8|issue=1|pages=574|doi=10.1038/msb.2012.6|issn=1744-4292|pmc=3321525|pmid=22395476}}</ref> The conception of the plant biological clock as made up of interacting negative feedback loops is unique in comparison to mammal and fungal circadian clocks which contain autoregulatory negative feedback loops with positive and negative elements<ref>{{Cite journal|last=Liu|first=Yi|last2=Bell-Pedersen|first2=Deborah|date=2017-04-27|title=Circadian Rhythms in Neurospora crassa and Other Filamentous Fungi|journal=Eukaryotic Cell|volume=5|issue=8|pages=1184–1193|doi=10.1128/EC.00133-06|issn=1535-9778|pmc=1539135|pmid=16896204}}</ref> (see "Transcriptional and non-transcriptional control on the [[Circadian clock]] page). |
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== Function and Interactions == |
== Function and Interactions == |
Revision as of 06:32, 2 May 2017
Pseudo-response regulator (PRR) refers to a group of genes that are important in the plant circadian oscillator. There are three primary PRR proteins (PRR9, PRR7, and PRR5) that perform the majority of interactions with other proteins within the circadian oscillator, and another (PRR3) that has limited function. These genes are all paralogs of each other, and all repress the transcription of Circadian Clock Associated 1 (CCA1) and Late Elongated Hypocotyl (LHY) at various times throughout the day. The expression of PRR9, PRR7, and PRR5 peak around morning, evening, and night, respectively. As a group, these genes are one part of the three-part repressilator system that governs the biological clock in plants.
Discovery
Multiple labs identified the PRR genes as parts of the circadian clock in the 1990s. In 2000, Akinori Matsushika, Seiya Makino, Masaya Kojima, and Takeshi Mizuno were the first to understand PRR genes as pseudo-response repressor genes rather than as response regulator (ARR) genes.[1][2] The factor that distinguishes PRR from ARR genes is the lack of a phospho-accepting aspartate site that characterizes ARR proteins. Though their research that discovered PRR genes was primarily hailed during the early 2000s as informing the scientific community about the function of TOC1, an additional pseudo-response regulator in the Arabidopsis thaliana biological clock,[3] the information about PRR genes that Matsushika and his team found deepened scientific understanding of circadian clocks in plants and led other researchers to hypothesize about the purpose of the PRR genes.[1] Though current research has identified PRR3, PRR5, PRR7, and PRR9 as of importance to the A. thaliana circadian clock mechanism, Matsushika et al. first categorized PRR genes into two subgroups (APRR1 and APRR2, the A stands for Arabidopsis) due to two differing amino acid structures.[4] The idea that the PRR genes formed parts of a complex repressilator of negative feedback loops was conceived by Andrew Millar’s lab in 2012.[5] The conception of the plant biological clock as made up of interacting negative feedback loops is unique in comparison to mammal and fungal circadian clocks which contain autoregulatory negative feedback loops with positive and negative elements[6] (see "Transcriptional and non-transcriptional control on the Circadian clock page).
Function and Interactions
PRR3, PRR5, PRR7 and PRR9 participate in the repressilator of a negative autoregulatory feedback loop that synchronizes to environmental inputs. The repressilator has a morning, evening, and night loop that are regulated in part by the pseudo-response regulator proteins' interactions with CCA1 and LHY. CCA1 and LHY exhibit peak binding to PRR9, PRR7, and PRR5 in the morning, evening, and night, respectively.[7]
PRR3 and PRR5
When phosphorylated by an unknown kinase, PRR5 and PRR3 demonstrate increased binding to Timing of Cab 1 ( TOC1). This interaction stabilizes both TOC1 and PRR5 and prevents their degradation by the F-box protein ZEITLUPE (ZTL).[7] Through this mechanism, PRR5 is indirectly activated by light, as ZTL is inhibited by light. Additionally, PRR5 contributes to the repression of the single MYB transcription factors CCA1 and LHY.[7]
PRR7 and PRR9
Two single MYB transcription factors, CCA1 and LHY, activate PRR7 and PRR 9. In turn, PRR7 and PRR9 repress CCA1 and LHY through the binding of their promoters. This interaction forms the morning loop of the repressilator of the biological clock in A. thaliana.[7] Chromatin immunoprecipitation demonstrates that LUX binds to the PRR9 promoter to repress it. Additionally, ELF3 has been shown to activate PRR9 and repress CCA1/LHY.[7] PRR9 is also activated by alternative RNA splicing. When PRMT5 (a methylation factor) is prevented from methylating intron 2 of PRR9, a frameshift resulting in premature truncation occurs.[7]
PRR7 and PRR9 also play a role in the entrainment of A. thaliana to a temperature-dependent cycle. Inactivation of PRR7 and PRR9 exhibits extreme period lengthening at high temperatures but shows no change at low temperatures. However, the inactivation of CCA1 and LHY in the PRR7/PRR9 loss-of-function mutants shows no change in period at high temperatures—this suggests that PRR7 and PRR9 are acting by overcompensation.[7]
Interactions Within Arabidopsis
In A. thaliana, the main feedback loop is proposed to involve a transcriptional regulation between several proteins. The three main components of this loop are TOC1 (also known as PRR1), CCA1 and LHY.[8] Each individual component peaks in transcriptions at different times of day.[9] PRR 9, 7 and 5 each significantly reduce the transcription levels of CCA1 and LHY.[9] In the opposite manner, PRR 9 and 7 slightly increase the transcription levels of TOC1.[9] The Constans (CO) is also indirectly regulated by the PRR proteins as well by setting up the molecular mechanism to dictate the photosensitive period in the afternoon.[10] PRRs are also known to stabilize CO at certain times of day to mediate its accumulation.[11] This results in the regulation of early flowering in shorter photoperiods, making light sensitivity and control of flowering time important functions of the PRR class.[10]
Homologs
Paralogs
PPR3, PRR5, PRR7, and PRR9 are all paralogs of each other. They have similar structure, and all repress the transcription of CCA1 and LHY. Additionally, they are all characterized by their lack of a phospho-accepting aspartate site. These genes are also paralogs to TOC1, which is alternatively called PRR1.[7]
Orthologs
Several pseudo-response regulators have been found in Selaginella, but their function has not yet been explored.[12]
Mutants
As PRR is a family of genes, several rounds mutant screening have been performed to identify each possible phenotype.
Rhythmicity Phenotype
In regards to rhythmicity of the clock in a free running setting PRR9 and PRR5 are associated with longer and shorter periods respectively.[9] For each gene, the double mutant with PRR7 exacerbates observed trends in rhythmicity.[9] The triple mutant renders the plant arrhythmic.[9]
Flowering Time Phenotype
In terms of flowering time in long day conditions, all mutants made the observed flowering late, with PRR7 significantly more late in comparison to the other mutants.[9] All double mutants with PRR7 saw much later flowering time than the PRR5/PRR9 mutant.[9]
Light Sensitivity Phenotype
With regard to light sensitivity, particularly in red light which is associated with hypocotyl lengthening, all PRR mutants were observed to be hypo-sensitive with PRR9 showing to be less sensitive.[9] All the double mutants were equal in hyposensitivity as the PRR5 or PRR7 mutants; the triple mutant is extremely hypo-sensitive.[9]
Future research
Recent research has showed that expression of clock genes show tissue-specificity.[13] Learning about how, when, and why specific tissues show certain peaks in clock genes like PRR can reveal more about the subtle nuances of each gene within the repressilator.
Few investigations into the circadian oscillator mechanisms in species other than A. thaliana have taken place; learning which genes are responsible for clock functions in other species will give more insight into the similarities and differences in clocks across plant species.[14]
The mechanistic details of each step in the plant biological clock repressilator system have yet to be fully understood. An understanding of these will give knowledge of clock function and, across species, increase understanding of the ecological and evolutionary functions of circadian oscillators.[7]
Additionally, identifying direct targets of PRR5, PRR7 and PRR9 that are not CCA1 and LHY will provide information about the molecular links from the PRRs to output genes like the flowering pathway and metabolism in mitochondria, which are CCA1-independent.[9]
See also
References
- ^ a b Salomé, Patrice A.; McClung, C. Robertson. "The Arabidopsis thaliana Clock". Journal of Biological Rhythms. 19 (5): 425–435. doi:10.1177/0748730404268112.
- ^ Boss, Paul K.; Bastow, Ruth M.; Mylne, Joshua S.; Dean, Caroline (2004-06-01). "Multiple Pathways in the Decision to Flower: Enabling, Promoting, and Resetting". The Plant Cell. 16 (suppl 1): S18–S31. doi:10.1105/tpc.015958. ISSN 1532-298X. PMC 2643402. PMID 15037730.
- ^ Alabadı́, David; Oyama, Tokitaka; Yanovsky, Marcelo J.; Harmon, Franklin G.; Más, Paloma; Kay, Steve A. (2001-08-03). "Reciprocal Regulation Between TOC1 and LHY/CCA1 Within the Arabidopsis Circadian Clock". Science. 293 (5531): 880–883. doi:10.1126/science.1061320. ISSN 0036-8075. PMID 11486091.
- ^ Matsushika, Akinori (2000). "Circadian Waves of Expression of the APRR1/TOC1 Family of Pseudo-Response Regulators in Arabidopsis thaliana: Insight into the Plant Circadian Clock". Plant Cell Physiology. 41: 1002–1012.
- ^ Pokhilko, Alexandra; Fernández, Aurora Piñas; Edwards, Kieron D.; Southern, Megan M.; Halliday, Karen J.; Millar, Andrew J. (2012-01-01). "The clock gene circuit in Arabidopsis includes a repressilator with additional feedback loops". Molecular Systems Biology. 8 (1): 574. doi:10.1038/msb.2012.6. ISSN 1744-4292. PMC 3321525. PMID 22395476.
- ^ Liu, Yi; Bell-Pedersen, Deborah (2017-04-27). "Circadian Rhythms in Neurospora crassa and Other Filamentous Fungi". Eukaryotic Cell. 5 (8): 1184–1193. doi:10.1128/EC.00133-06. ISSN 1535-9778. PMC 1539135. PMID 16896204.
- ^ a b c d e f g h i McClung, C. Robertson (2006-04-01). "Plant Circadian Rhythms". The Plant Cell. 18 (4): 792–803. doi:10.1105/tpc.106.040980. ISSN 1532-298X. PMC 1425852. PMID 16595397.
- ^ Alabadi, David; Oyama, Tokitaka; Yanovsky, Marcelo; Harmon, Franklin (2001). "Reciprocal regulation between TOC1 and LHY/CCA 1 within the Arabidopsis Circadian clock". Science. 293: 880–883.
- ^ a b c d e f g h i j k Nakamichi, Norihito; Kiba, Takatoshi; Henriques, Rossana; Mizuno, Takeshi; Chua, Nam-Hai; Sakakibara, Hitoshi (2010-03-01). "PSEUDO-RESPONSE REGULATORS 9, 7, and 5 Are Transcriptional Repressors in the Arabidopsis Circadian Clock". The Plant Cell. 22 (3): 594–605. doi:10.1105/tpc.109.072892. ISSN 1532-298X. PMC 2861452. PMID 20233950.
- ^ a b Golembeski, Greg S.; Imaizumi, Takato (2015-01-01). "Photoperiodic Regulation of Florigen Function in Arabidopsis thaliana". The Arabidopsis Book: e0178. doi:10.1199/tab.0178. PMC 4489636. PMID 26157354.
- ^ Hayama, Ryosuke; Sarid-Krebs, Liron; Richter, René; Fernández, Virginia; Jang, Seonghoe; Coupland, George (3 April 2017). "PSEUDO RESPONSE REGULATORs stabilize CONSTANS protein to promote flowering in response to day length". The EMBO journal. 36 (7): 904–918. doi:10.15252/embj.201693907. ISSN 1460-2075. PMID 28270524.
- ^ Banks, Jo Ann; Nishiyama, Tomoaki; Hasebe, Mitsuyasu; Bowman, John L.; Gribskov, Michael; dePamphilis, Claude; Albert, Victor A.; Aono, Naoki; Aoyama, Tsuyoshi (2011-05-20). "The compact Selaginella genome identifies changes in gene content associated with the evolution of vascular plants". Science (New York, N.Y.). 332 (6032): 960–963. doi:10.1126/science.1203810. ISSN 0036-8075. PMC 3166216. PMID 21551031.
- ^ Shim, Jae Sung; Kubota, Akane; Imaizumi, Takato (2017-01-01). "Circadian Clock and Photoperiodic Flowering in Arabidopsis: CONSTANS Is a Hub for Signal Integration". Plant Physiology. 173 (1): 5–15. doi:10.1104/pp.16.01327. ISSN 1532-2548. PMC 5210731. PMID 27688622.
- ^ Shim, Jae Shung; Imaizumi, Takato (2014). "Circadian Clock and Photoperiodic Response in Arabidopsis: From Seasonal Flowering to Redox Homeostasis". Biochemistry. 54: 157–170.