PIN domain: Difference between revisions
Surykartka (talk | contribs) Added a reference |
Surykartka (talk | contribs) Added references in the text and small corrections about molecular function. |
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In molecular biology the '''PIN domain''' is a [[protein domain]] that is about 130 amino acids in length |
In molecular biology the '''PIN domain''' is a [[protein domain]] that is about 130 amino acids in length. PIN domains function as [[nuclease]] enzymes that cleave [[ssRNA|single stranded RNA]] in a sequence- or structure-dependent manner.<ref name="pmid21036780">{{cite journal |vauthors=Arcus VL, McKenzie JL, Robson J, Cook GM |title=The PIN-domain ribonucleases and the prokaryotic VapBC toxin-antitoxin array |journal=Protein Eng. Des. Sel. |volume=24 |issue=1-2 |pages=33–40 |date=January 2011 |pmid=21036780 |doi=10.1093/protein/gzq081 |url=}}</ref><ref name=":0" /> |
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PIN domains contain |
PIN domains contain four nearly invariant acidic residues.<ref name=":0" /> Crystal structures show these residues clustered together in the putative [[active site]]. In eukaryotes PIN domains are found in proteins involved in [[nonsense mediated decay|nonsense mediated mRNA decay]], in proteins such as [[SMG5]] and [[SMG6]], and in processing of 18S [[ribosomal RNA]]. The majority of PIN-domain proteins found in prokaryotes are the toxic components of [[toxin-antitoxin system|toxin-antitoxin operon]]s.<ref name=":0">{{Cite journal|last=Matelska|first=D|last2=Steczkiewicz|first2=K|last3=Ginalski|first3=K|date=2017-07-07|title=Comprehensive classification of the PIN domain-like superfamily|url=https://academic.oup.com/nar/article-lookup/doi/10.1093/nar/gkx494|journal=Nucleic Acids Research|language=en|volume=45|issue=12|pages=6995–7020|doi=10.1093/nar/gkx494|issn=0305-1048|pmc=PMC5499597|pmid=28575517|via=}}</ref> These loci provide a control mechanism that helps free-living prokaryotes cope with nutritional stress.<ref name="pmid15864262">{{cite journal |vauthors=Gerdes K, Christensen SK, Løbner-Olesen A |title=Prokaryotic toxin-antitoxin stress response loci |journal=Nat. Rev. Microbiol. |volume=3 |issue=5 |pages=371–82 |date=May 2005 |pmid=15864262 |doi=10.1038/nrmicro1147 |url=}}</ref> |
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==References== |
==References== |
Revision as of 22:00, 25 March 2019
PIN domain | |||||||||||
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![]() Crystal structure of PIN (PilT N-terminus) domain (AF0591) from Archaeoglobus fulgidus at 1.90 Angstrom resolution. 1o4w | |||||||||||
Identifiers | |||||||||||
Symbol | PIN | ||||||||||
Pfam | PF01850 | ||||||||||
InterPro | IPR002716 | ||||||||||
SMART | CBS | ||||||||||
SCOP2 | 3dbo / SCOPe / SUPFAM | ||||||||||
CDD | cd09852 | ||||||||||
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In molecular biology the PIN domain is a protein domain that is about 130 amino acids in length. PIN domains function as nuclease enzymes that cleave single stranded RNA in a sequence- or structure-dependent manner.[1][2]
PIN domains contain four nearly invariant acidic residues.[2] Crystal structures show these residues clustered together in the putative active site. In eukaryotes PIN domains are found in proteins involved in nonsense mediated mRNA decay, in proteins such as SMG5 and SMG6, and in processing of 18S ribosomal RNA. The majority of PIN-domain proteins found in prokaryotes are the toxic components of toxin-antitoxin operons.[2] These loci provide a control mechanism that helps free-living prokaryotes cope with nutritional stress.[3]
References
- ^ Arcus VL, McKenzie JL, Robson J, Cook GM (January 2011). "The PIN-domain ribonucleases and the prokaryotic VapBC toxin-antitoxin array". Protein Eng. Des. Sel. 24 (1–2): 33–40. doi:10.1093/protein/gzq081. PMID 21036780.
- ^ a b c Matelska, D; Steczkiewicz, K; Ginalski, K (2017-07-07). "Comprehensive classification of the PIN domain-like superfamily". Nucleic Acids Research. 45 (12): 6995–7020. doi:10.1093/nar/gkx494. ISSN 0305-1048. PMC 5499597. PMID 28575517.
{{cite journal}}
: CS1 maint: PMC format (link) - ^ Gerdes K, Christensen SK, Løbner-Olesen A (May 2005). "Prokaryotic toxin-antitoxin stress response loci". Nat. Rev. Microbiol. 3 (5): 371–82. doi:10.1038/nrmicro1147. PMID 15864262.