Abstract
Protein complexes of the SWI/SNF family remodel nucleosome structure in an ATP-dependent manner. Each complex contains between 8 and 15 subunits, several of which are highly conserved between yeast, Drosophila, and humans. We have reconstituted an ATP-dependent chromatin remodeling complex using a subset of conserved subunits. Unexpectedly, both BRG1 and hBRM, the ATPase subunits of human SWI/SNF complexes, are capable of remodeling mono-nucleosomes and nucleosomal arrays as purified proteins. The addition of INI1, BAF155, and BAF170 to BRG1 increases remodeling activity to a level comparable to that of the whole hSWI/SNF complex. These data define the functional core of the hSWI/SNF complex.
Publication types
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Research Support, Non-U.S. Gov't
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Actins / chemistry*
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Actins / physiology
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Adenosine Triphosphate / physiology
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Cell-Free System
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Chromatin / metabolism
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Chromatin / ultrastructure*
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Chromosomal Proteins, Non-Histone*
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DNA Helicases
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DNA-Binding Proteins / chemistry*
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DNA-Binding Proteins / physiology
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Drosophila Proteins*
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Evolution, Molecular
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Humans
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Macromolecular Substances
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Nuclear Proteins / chemistry*
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Nuclear Proteins / physiology
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Nucleosomes / metabolism
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Nucleosomes / ultrastructure*
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RNA-Binding Proteins*
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Recombinant Fusion Proteins / chemistry
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Ribonucleoprotein, U1 Small Nuclear / chemistry*
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Ribonucleoprotein, U1 Small Nuclear / physiology
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Transcription Factors / chemistry*
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Transcription Factors / physiology
Substances
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ACTL6A protein, human
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Actins
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Chromatin
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Chromosomal Proteins, Non-Histone
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DNA-Binding Proteins
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Drosophila Proteins
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Macromolecular Substances
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Nuclear Proteins
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Nucleosomes
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RNA-Binding Proteins
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Recombinant Fusion Proteins
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Ribonucleoprotein, U1 Small Nuclear
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SMARCA2 protein, human
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SMARCC1 protein, human
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SMARCC2 protein, human
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SMARCD1 protein, human
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Transcription Factors
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snf protein, Drosophila
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Adenosine Triphosphate
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SMARCA4 protein, human
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DNA Helicases