Microbial epitopes act as altered peptide ligands to prevent experimental autoimmune encephalomyelitis

J Exp Med. 1999 Apr 19;189(8):1275-84. doi: 10.1084/jem.189.8.1275.

Abstract

Molecular mimicry refers to structural homologies between a self-protein and a microbial protein. A major epitope of myelin basic protein (MBP), p87-99 (VHFFKNIVTPRTP), induces experimental autoimmune encephalomyelitis (EAE). VHFFK contains the major residues for binding of this self-molecule to T cell receptor (TCR) and to the major histocompatibility complex. Peptides from papilloma virus strains containing the motif VHFFK induce EAE. A peptide from human papilloma virus type 40 (HPV 40) containing VHFFR, and one from HPV 32 containing VHFFH, prevented EAE. A sequence from Bacillus subtilis (RKVVTDFFKNIPQRI) also prevented EAE. T cell lines, producing IL-4 and specific for these microbial peptides, suppressed EAE. Thus, microbial peptides, differing from the core motif of the self-antigen, MBPp87-99, function as altered peptide ligands, and behave as TCR antagonists, in the modulation of autoimmune disease.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Bacterial Proteins / immunology*
  • Cell Division / immunology
  • Encephalomyelitis, Autoimmune, Experimental / immunology*
  • Encephalomyelitis, Autoimmune, Experimental / prevention & control
  • Epitopes / immunology*
  • Female
  • Interferon-gamma / metabolism
  • Interleukin-4 / metabolism
  • Ligands*
  • Mice
  • Mice, Inbred Strains
  • Molecular Sequence Data
  • Myelin Basic Protein / immunology
  • Peptide Fragments / immunology*
  • Receptors, Antigen, T-Cell / antagonists & inhibitors
  • T-Lymphocytes / immunology*

Substances

  • Bacterial Proteins
  • Epitopes
  • Ligands
  • Myelin Basic Protein
  • Peptide Fragments
  • Receptors, Antigen, T-Cell
  • myelin basic protein 85-99
  • myelin basic protein 87-99
  • Interleukin-4
  • Interferon-gamma