Abstract
An ELISA method based on monodisperse magnetic beads was developed for the detection of Staphylococcus aureus thermostable nuclease (TNase) in composite milk, wherein S. aureus TNase is captured by magnetic beads coated with monoclonal antibodies directed against TNase and subsequently detected by an enzyme-labelled MAb against the same antigen. Sensitivity of the test was approximately 1 ng TNase, which corresponds to the amount of TNase produced and secreted by approximately 10(5) S. aureus per ml. The Immuno Magnetic Separation (IMS)-ELISA detected TNase in samples from which no S. aureus could be demonstrated on culture. The total test time is 3 h and can be performed either on preserved or fresh milk. The method may be automated.
Publication types
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Comparative Study
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Research Support, Non-U.S. Gov't
MeSH terms
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Animals
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Antibodies, Monoclonal
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Cattle
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Coloring Agents / chemistry
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Enzyme-Linked Immunosorbent Assay / methods
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Enzyme-Linked Immunosorbent Assay / veterinary
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Female
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Immunomagnetic Separation / methods
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Immunomagnetic Separation / veterinary
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Mastitis, Bovine / diagnosis*
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Mastitis, Bovine / immunology
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Micrococcal Nuclease / analysis
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Micrococcal Nuclease / chemistry
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Microspheres
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Milk / microbiology
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Sensitivity and Specificity
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Staphylococcal Infections / diagnosis
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Staphylococcal Infections / immunology
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Staphylococcal Infections / veterinary*
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Staphylococcus aureus / immunology
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Staphylococcus aureus / isolation & purification*
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Tolonium Chloride / chemistry
Substances
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Antibodies, Monoclonal
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Coloring Agents
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Tolonium Chloride
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Micrococcal Nuclease