Abstract
In cholinergic neurons, high-affinity choline uptake in presynaptic terminals is the rate-limiting step in acetylcholine synthesis. Using information provided by the Caenorhabditis elegans Genome Project, we cloned a cDNA encoding the high-affinity choline transporter from C. elegans (cho-1). We subsequently used this clone to isolate the corresponding cDNA from rat (CHT1). CHT1 is not homologous to neurotransmitter transporters, but is homologous to members of the Na+-dependent glucose transporter family. Expression of CHT1 mRNA is restricted to cholinergic neurons. The characteristics of CHT1-mediated choline uptake essentially match those of high-affinity choline uptake in rat brain synaptosomes.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Amino Acid Sequence
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Animals
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Binding, Competitive / drug effects
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CHO Cells
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Caenorhabditis elegans
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Carrier Proteins / chemistry*
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Carrier Proteins / genetics*
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Carrier Proteins / metabolism
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Cell Membrane / metabolism
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Choline / metabolism
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Choline / pharmacokinetics
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Cloning, Molecular
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Cricetinae
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DNA, Complementary / genetics
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Hemicholinium 3 / pharmacology
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Membrane Transport Proteins*
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Molecular Sequence Data
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Monosaccharide Transport Proteins / genetics
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Neurons / metabolism
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Neurotransmitter Uptake Inhibitors / pharmacology
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Oocytes / cytology
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Oocytes / metabolism
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Organ Specificity
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Phylogeny
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RNA, Messenger / biosynthesis
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Rats
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Sequence Homology, Amino Acid
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Sodium / metabolism
Substances
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Carrier Proteins
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DNA, Complementary
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Membrane Transport Proteins
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Monosaccharide Transport Proteins
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Neurotransmitter Uptake Inhibitors
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RNA, Messenger
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choline transporter
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Hemicholinium 3
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Sodium
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Choline