Abstract
We have purified apical merozoite antigen 1 (AMA-1) from extracts of red blood cells infected with the rodent malaria parasite Plasmodium yoelii yoelii YM. When used to immunize mice, the protein induced a strong protective response against a challenge with the parasite. Monoclonal antibodies specific for P. yoelii yoelii AMA-1 were prepared, and one was very effective against the parasite on passive immunization. A second protein that appears to be located in the apical rhoptry organelles and associated with AMA-1 was identified.
MeSH terms
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Animals
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Antibodies, Monoclonal / immunology
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Antibodies, Protozoan / immunology*
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Antibody Specificity
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Antigens, Protozoan / immunology*
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Antigens, Protozoan / isolation & purification
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Antigens, Surface / immunology*
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Antigens, Surface / isolation & purification
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Cross Reactions
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Female
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Fluorescent Antibody Technique
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Immunization, Passive / methods
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Immunoblotting
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Malaria / prevention & control*
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Malaria Vaccines / immunology*
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Membrane Proteins / immunology*
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Membrane Proteins / isolation & purification
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Mice
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Mice, Inbred BALB C
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Plasmodium falciparum / immunology
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Plasmodium yoelii / immunology*
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Precipitin Tests
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Protozoan Proteins / immunology*
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Protozoan Proteins / isolation & purification
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Vaccination / methods
Substances
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Antibodies, Monoclonal
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Antibodies, Protozoan
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Antigens, Protozoan
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Antigens, Surface
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Malaria Vaccines
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Membrane Proteins
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Protozoan Proteins
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apical membrane antigen I, Plasmodium