Abstract
Objective:
To see how arsenic trioxide (As2O3) affects proliferation of the human B lymphoma cell line MBC-1.
Methods:
We studied the effect of As2O3 on MBC-1 cells and its mechanism by morphological observation, flow cytometry assay and DNA gel electrophoresis.
Results:
As2O3 could upregulate p53 gene expression at protein level, inducing cell apoptosis and inhibiting the proliferation of MBC-1 cells. Upregulation of p53 expression appears to be important in the apoptosis of MBC-1 cells.
Conclusions:
As2O3 can inhibit the proliferation of MBC-1 cells by upregulating p53 gene expression, thus inducing apoptosis.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Antineoplastic Agents / pharmacology*
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Apoptosis / drug effects*
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Arsenic Trioxide
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Arsenicals / pharmacology*
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Burkitt Lymphoma / pathology*
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Cell Division / drug effects
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DNA Damage
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DNA Fragmentation
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Gene Expression Regulation, Neoplastic / drug effects*
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Genes, bcl-2
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Genes, p53
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Humans
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Neoplasm Proteins / biosynthesis
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Neoplasm Proteins / genetics
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Oxides / pharmacology*
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Proto-Oncogene Proteins c-bcl-2 / biosynthesis
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Tumor Cells, Cultured / drug effects
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Tumor Suppressor Protein p53 / biosynthesis
Substances
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Antineoplastic Agents
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Arsenicals
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Neoplasm Proteins
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Oxides
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Proto-Oncogene Proteins c-bcl-2
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Tumor Suppressor Protein p53
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Arsenic Trioxide