Epitope mapping on N-terminal region of taenia solium paramyosin

Immunol Lett. 2000 Jun 1;72(3):191-5. doi: 10.1016/s0165-2478(00)00189-9.

Abstract

Epitope mapping of the amino-terminal 20aa sequence from Taenia solium paramyosin (TPmy), an immunodominant protein involved in the complex host-parasite relationship in human and porcine cysticercosis is reported. A 12-mer random peptide phage display library was screened with antibodies raised against a synthetic peptide corresponding to the amino-terminal 20aa sequence of TPmy, its highly immunodominant region. In total, 57 clones isolated in two panning conditions were analyzed, of which a single group of 14 sequences found in 25 clones shared a consensus motif showing structural similarity with the antigen Arg10-Thr16 region.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Epitope Mapping / methods
  • Epitopes, B-Lymphocyte / immunology*
  • Immunodominant Epitopes / immunology*
  • Molecular Sequence Data
  • Peptides / immunology
  • Rabbits
  • Taenia / immunology*
  • Tropomyosin / immunology*

Substances

  • Epitopes, B-Lymphocyte
  • Immunodominant Epitopes
  • Peptides
  • Tropomyosin