Abstract
A clinical strain of Escherichia coli (Ec GCE) displayed resistance to cefoxitin, cefotetan, cefotaxime, and ceftazidime. Susceptibility was not restored by the addition of clavulanic acid. Two beta-lactamases with apparent pIs of 5.4 and 6.4 were identified; the beta-lactamase with a pI of 6.4 was transferred by conjugation and associated with a 40-kb plasmid. Analysis of the nucleotide sequence showed a new ampC beta-lactamase gene that is closely related to those encoding the FOX-3, FOX-2, and FOX-1 beta-lactamases but whose product has four novel amino acid mutations, at positions 11 (M-->T), 43 (A-->E), 233 (V-->A), and 280 (Y-->H). This first cephamycinase from Spain was named FOX-4.
MeSH terms
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Amino Acid Sequence
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Anti-Bacterial Agents / pharmacology
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Bacterial Proteins*
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Base Sequence
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Cloning, Molecular
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DNA, Bacterial / analysis
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Drug Resistance, Microbial / genetics
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Escherichia coli / drug effects
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Escherichia coli / enzymology
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Escherichia coli / genetics*
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Escherichia coli Proteins
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Humans
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Hydrolysis
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Isoelectric Focusing
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Kinetics
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Molecular Sequence Data
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Plasmids / genetics
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Sequence Analysis, DNA
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Sequence Homology, Amino Acid
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Spanien
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beta-Lactamases / chemistry
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beta-Lactamases / drug effects
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beta-Lactamases / genetics*
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beta-Lactams
Substances
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Anti-Bacterial Agents
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Bacterial Proteins
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DNA, Bacterial
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Escherichia coli Proteins
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beta-Lactams
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fox-4 protein, E coli
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AmpC beta-lactamases
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beta-Lactamases