The domineering non-autonomy of frizzled and van Gogh clones in the Drosophila wing is a consequence of a disruption in local signaling

Mech Dev. 2000 Sep;96(2):197-207. doi: 10.1016/s0925-4773(00)00392-0.

Abstract

The frizzled (fz) gene is required for the development of distally pointing hairs on the Drosophila wing. It has been suggested that fz is needed for the propagation of a signal along the proximal distal axis of the wing. The directional domineering non-autonomy of fz clones could be a consequence of a failure in the propagation of this signal. We have tested this hypothesis in two ways. In one set of experiments we used the domineering non-autonomy of fz and Vang Gogh (Vang) clones to assess the direction of planar polarity signaling in the wing. prickle (pk) mutations alter wing hair polarity in a cell autonomous way, so pk cannot be altering a global polarity signal. However, we found that pk mutations altered the direction of the domineering non-autonomy of fz and Vang clones, arguing that this domineering non-autonomy is not due to an alteration in a global signal. In a second series of experiments we ablated cells in the pupal wing. We found that a lack of cells that could be propagating a long-range signal did not alter hair polarity. We suggest that fz and Vang clones result in altered levels of a locally acting signal and the domineering non-autonomy results from wild-type cells responding to this abnormal signal.

Publication types

  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Alleles
  • Animals
  • Body Patterning / genetics
  • DNA-Binding Proteins / genetics
  • Drosophila / genetics*
  • Drosophila / growth & development*
  • Drosophila Proteins*
  • Frizzled Receptors
  • Genes, Insect*
  • LIM Domain Proteins
  • Membrane Proteins / genetics*
  • Models, Biological
  • Mosaicism
  • Mutation
  • Pupa / growth & development
  • Receptors, G-Protein-Coupled
  • Signal Transduction
  • Wings, Animal / growth & development*

Substances

  • DNA-Binding Proteins
  • Drosophila Proteins
  • Frizzled Receptors
  • LIM Domain Proteins
  • Membrane Proteins
  • Receptors, G-Protein-Coupled
  • fz protein, Drosophila
  • pk protein, Drosophila