Bioanalytical applications of 'fast chromatography' to high-throughput liquid chromatography/tandem mass spectrometric quantitation

Rapid Commun Mass Spectrom. 2000;14(18):1662-8. doi: 10.1002/1097-0231(20000930)14:18<1662::AID-RCM77>3.0.CO;2-N.

Abstract

A fast chromatographic separation approach that enables rapid method development for high-throughput sample quantification is discussed. This approach has been used to analyze samples from various biological matrices. Data are presented from in vivo pharmacokinetic studies (plasma) and in vitro drug metabolism and transport studies (hepatic microsomes, hepatocytes, and Caco-2 cells).

MeSH terms

  • Animals
  • Biological Transport
  • Brain Chemistry
  • Caco-2 Cells
  • Chromatography, High Pressure Liquid / methods*
  • Humans
  • In Vitro Techniques
  • Liver / metabolism
  • Mass Spectrometry / methods*
  • Microsomes, Liver / metabolism
  • Particle Size
  • Pharmacokinetics
  • Rats