Association of protease activity in Vibrio cholerae vaccine strains with decreases in transcellular epithelial resistance of polarized T84 intestinal epithelial cells

Infect Immun. 2000 Nov;68(11):6487-92. doi: 10.1128/IAI.68.11.6487-6492.2000.

Abstract

Culture supernatants prepared from reactogenic strains of Vibrio cholerae cause a decrease in the transcellular epithelial resistance of T84 intestinal cells. This decrease correlates with the presence of hemagglutinin/protease but not with the presence of other potential accessory toxins or proteases. These data suggest a possible role for hemagglutinin/protease in reactogenicity, although other factors may also contribute.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Aminopeptidases / physiology
  • Cell Line
  • Cell Polarity
  • Cholera Toxin / biosynthesis
  • Cholera Vaccines*
  • Endopeptidases / physiology*
  • Endotoxins
  • Hemagglutinins / physiology
  • Intestinal Mucosa / physiology*
  • Vibrio cholerae / physiology*

Substances

  • Cholera Vaccines
  • Endotoxins
  • Hemagglutinins
  • zonula occludens toxin, Vibrio cholerae
  • Cholera Toxin
  • Endopeptidases
  • Aminopeptidases