Abstract
The expression of two components of the glucose-6-phosphatase system, the catalytic subunit (G6PaseC) and the glucose-6-phosphate transporter, was analyzed in the clear cell type of human renal cell carcinoma. The expression of G6PaseC was decreased in tumours compared with non-tumourous tissue of the same patient. The expression of G6PaseT varied with no general trend between tumours and control tissue. The expression of protein kinase B (PKB) was unchanged in the tumours, suggesting that the down-regulation of G6PaseC in clear cells and the maintenance of the transformed phenotype are not predominantly caused by an overexpression of PKB.
MeSH terms
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Aged
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Antiporters / biosynthesis*
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Antiporters / genetics
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Blotting, Northern
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Carcinoma, Renal Cell / enzymology*
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Carcinoma, Renal Cell / genetics
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Catalytic Domain / genetics
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Down-Regulation / physiology
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Female
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Gene Expression Regulation, Enzymologic / physiology
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Gene Expression Regulation, Neoplastic / physiology
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Glucose-6-Phosphatase / antagonists & inhibitors
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Glucose-6-Phosphatase / biosynthesis*
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Glucose-6-Phosphatase / genetics
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Humans
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Kidney Neoplasms / enzymology*
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Kidney Neoplasms / genetics
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Male
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Middle Aged
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Monosaccharide Transport Proteins / biosynthesis*
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Monosaccharide Transport Proteins / genetics
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Protein Serine-Threonine Kinases*
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Proto-Oncogene Proteins / biosynthesis*
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Proto-Oncogene Proteins / genetics
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Proto-Oncogene Proteins c-akt
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Reverse Transcriptase Polymerase Chain Reaction
Substances
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Antiporters
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Monosaccharide Transport Proteins
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Proto-Oncogene Proteins
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SLC37A4 protein, human
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glucose 6-phosphate(transporter)
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Protein Serine-Threonine Kinases
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Proto-Oncogene Proteins c-akt
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Glucose-6-Phosphatase