Abstract
The t(14;18) translocation is a useful marker to characterize follicular lymphoma and to monitor residual disease. The polymerase chain reaction (PCR) is a powerful technique to detect this translocation. Located on chromosome 18, within the Bcl-2 gene, breakpoints occur mainly in the 3; untranslated region, in the third exon of Bcl-2 (MBR region). In this study, the authors amplified MBR breakpoints by PCR and found an unexpectedly large fragment of 1 Kb that corresponds to a recently described new breakpoint in the Bcl-2 gene. With a new primer set, a further previously considered t(14;18)-unrelated tumor was in fact positive for this new breakpoint.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Base Sequence
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Blotting, Southern
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Chromosome Breakage
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Chromosomes, Human, Pair 14*
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Chromosomes, Human, Pair 18*
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DNA Primers / chemistry
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DNA, Neoplasm / analysis
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Electrophoresis, Agar Gel
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Genes, bcl-2 / genetics*
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Humans
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Immunoenzyme Techniques
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Lymph Nodes / chemistry
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Lymph Nodes / pathology
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Lymphoma, Follicular / chemistry
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Lymphoma, Follicular / genetics*
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Lymphoma, Follicular / pathology
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Molecular Sequence Data
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Oligonucleotide Probes / chemistry
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Polymerase Chain Reaction / methods*
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Proto-Oncogene Proteins c-bcl-2 / analysis
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Translocation, Genetic / genetics*
Substances
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DNA Primers
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DNA, Neoplasm
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Oligonucleotide Probes
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Proto-Oncogene Proteins c-bcl-2