Use of cultured cells in assessing ethanol toxicity and ethanol-related metabolism

Alcohol Clin Exp Res. 2001 May;25(5 Suppl ISBRA):87S-93S. doi: 10.1097/00000374-200105051-00016.

Abstract

This article represents the proceedings of a symposium at the 2000 ISBRA Meeting in Yokohama, Japan. The chairs were Terrence M. Donohue, Jr, and Dahn L. Clemens. The presentations were (1) Characterization of single and double recombinant hepatoma cells that express ethanol-metabolizing enzymes, by Terrence M. Donohue, Jr; (2) Inhibition of cell growth by ethanol metabolism, by Dahn L. Clemens; (3) Use of transfected HeLa cells to study the genesis of alcoholic fatty liver, by Andrea Galli and David Crabb; (4) CYP2E1-mediated oxidative stress induces COL1A2 mRNA in hepatic stellate cells and in a coculture system of HepG2 and stellate cells, by Natalia Nieto; (5) Transforming growth factor-alpha secreted from ethanol-exposed hepatocytes contributes to development of alcoholic hepatic fibrosis, by Junji Kato; and (6) Effect of ethanol on Fas-dependent caspase-3 activation and apoptosis in CD4+ T cells, by Shirish S. Barve.

MeSH terms

  • Acetaldehyde / metabolism
  • Alcohol Dehydrogenase / drug effects
  • Alcohol Dehydrogenase / metabolism
  • Animals
  • Apoptosis / drug effects*
  • Apoptosis / physiology
  • CD4-Positive T-Lymphocytes / drug effects
  • CD4-Positive T-Lymphocytes / metabolism*
  • Cell Division / drug effects
  • Cell Division / physiology
  • Central Nervous System Depressants / metabolism
  • Central Nervous System Depressants / toxicity*
  • Cytochrome P-450 CYP2E1 / drug effects
  • Cytochrome P-450 CYP2E1 / metabolism
  • Ethanol / metabolism
  • Ethanol / toxicity*
  • Fatty Liver, Alcoholic / metabolism*
  • HeLa Cells / drug effects
  • Hepatocytes / drug effects*
  • Hepatocytes / metabolism
  • Humans
  • Rats
  • Transforming Growth Factor alpha / drug effects
  • Transforming Growth Factor alpha / metabolism

Substances

  • Central Nervous System Depressants
  • Transforming Growth Factor alpha
  • Ethanol
  • Alcohol Dehydrogenase
  • Cytochrome P-450 CYP2E1
  • Acetaldehyde