Abstract
To characterize the immunological effects of intermittent IL-2 therapy, which leads to selective increases in CD4+ T lymphocytes in HIV-infected patients, 11 patients underwent extensive immunological evaluation. While IL-2 induced changes in both CD4+ and CD8+ cell number acutely, only CD4+ cells showed sustained increases following discontinuation of IL-2. Transient increases in expression of the activation markers CD38 and HLA-DR were seen on both CD4+ and CD8+ cells, but CD25 (a chain of the IL-2 receptor) increased exclusively on CD4+ cells. This increase in CD25 expression was sustained for months following discontinuation of IL-2, and was seen in naive as well as memory cells. IL-2 induced cell proliferation, but tachyphylaxis to these proliferative effects developed after 1 week despite continued IL-2 administration. It thus appears that sustained CD25 expression selectively on CD4+ cells is a critical component of the immunological response to IL-2, and that intermittent administration of IL-2 is necessary to overcome the tachyphylaxis to IL-2-induced proliferation.
Publication types
-
Clinical Trial
-
Research Support, U.S. Gov't, P.H.S.
MeSH terms
-
ADP-ribosyl Cyclase
-
ADP-ribosyl Cyclase 1
-
Adult
-
Antigens, CD*
-
Antigens, Differentiation / metabolism
-
CD4-CD8 Ratio
-
CD4-Positive T-Lymphocytes / drug effects
-
CD4-Positive T-Lymphocytes / immunology
-
CD4-Positive T-Lymphocytes / metabolism
-
Cell Division / drug effects
-
Flow Cytometry
-
HIV Infections / drug therapy*
-
HIV Infections / immunology*
-
HLA-DR Antigens / metabolism
-
Humans
-
Immunologic Memory / immunology
-
Immunotherapy*
-
Interleukin-2 / administration & dosage
-
Interleukin-2 / immunology*
-
Interleukin-2 / pharmacology
-
Interleukin-2 / therapeutic use*
-
Lymphocyte Activation / drug effects
-
Lymphocyte Subsets / drug effects
-
Lymphocyte Subsets / immunology
-
Lymphocyte Subsets / metabolism
-
Male
-
Membrane Glycoproteins
-
NAD+ Nucleosidase / metabolism
-
Receptors, Interleukin-2 / metabolism
-
Tachyphylaxis
-
Time Factors
Substances
-
Antigens, CD
-
Antigens, Differentiation
-
HLA-DR Antigens
-
Interleukin-2
-
Membrane Glycoproteins
-
Receptors, Interleukin-2
-
ADP-ribosyl Cyclase
-
CD38 protein, human
-
NAD+ Nucleosidase
-
ADP-ribosyl Cyclase 1