Characterization and protective potential of the immune response to Taenia solium paramyosin in a murine model of cysticercosis

Infect Immun. 2001 Sep;69(9):5412-6. doi: 10.1128/IAI.69.9.5412-5416.2001.

Abstract

Paramyosin has been proposed as a vaccine candidate in schistosomiasis and filariasis. However, limited information is available about its protective potential against cysticercosis and the immune response it induces. Immunization of mice with recombinant full-length paramyosin of Taenia solium (TPmy) results in about a 52% reduction in parasite burden after a subsequent challenge by intraperitoneal inoculation of Taenia crassiceps cysticerci. Immunization assays using recombinant fragments of TPmy, corresponding approximately to thirds on the amino, central, or carboxyl regions, suggest that protective epitopes are located mostly in the amino-end third. Proliferation assays using T cells obtained from mice immunized with the full-length recombinant TPmy also showed a preferential response to the amino-terminal fragment. In contrast, antibodies in the sera from these mice predominantly recognize epitopes located in the carboxyl-terminal fragment, being the immunoglobulin G1 subclass, the predominant antibody isotype. Characterization of the cellular immune response induced against the protective amino-terminal fragment reveals production of gamma interferon and interleukin-2, but not interleukin-4, suggesting a Th1-like profile.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Antibodies, Helminth / blood
  • Cysticercosis / immunology*
  • Cysticercosis / parasitology
  • Cysticercosis / prevention & control*
  • Cytokines / biosynthesis
  • Disease Models, Animal
  • Female
  • Lymphocyte Activation
  • Mice
  • Mice, Inbred BALB C
  • Recombinant Proteins / genetics
  • Recombinant Proteins / immunology
  • T-Lymphocytes / immunology
  • Taenia / immunology*
  • Tropomyosin / genetics
  • Tropomyosin / immunology*
  • Vaccination
  • Vaccines / immunology*

Substances

  • Antibodies, Helminth
  • Cytokines
  • Recombinant Proteins
  • Tropomyosin
  • Vaccines