Abstract
Single-chain antibodies neutralize activity and bind nonoverlapping epitopes of botulinum A neurotoxin. Two phage display epitope libraries were constructed from the 1.3 kb of binding domain cDNA. The minimal epitopes selected against the single-chain Fv-Fc antibodies correspond to conformational epitopes with amino acid residues 1115 to 1223 (S25), 1131 to 1264 (3D12), and 889 to 1294 (C25).
Publication types
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Research Support, U.S. Gov't, Non-P.H.S.
MeSH terms
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Animals
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Antibodies, Bacterial / immunology*
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Botulinum Toxins, Type A / chemistry
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Botulinum Toxins, Type A / genetics
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Botulinum Toxins, Type A / immunology*
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Clostridium botulinum / immunology*
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Epitope Mapping / methods
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Epitopes, B-Lymphocyte / chemistry
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Epitopes, B-Lymphocyte / genetics
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Epitopes, B-Lymphocyte / immunology*
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Humans
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Immunoglobulin Fragments / immunology*
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Immunoglobulin Variable Region / immunology*
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Mice
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Models, Molecular
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Neutralization Tests
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Peptide Library
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Protein Structure, Tertiary
Substances
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Antibodies, Bacterial
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Epitopes, B-Lymphocyte
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Immunoglobulin Fragments
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Immunoglobulin Variable Region
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Peptide Library
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immunoglobulin Fv
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Botulinum Toxins, Type A