Objective: To secure anti-endotheliocyte hyperplasia functional fragment of human angiostatin K (1-3) gene.
Methods: As the template of leukocytotic cDNA, functional fragment of human angiostatin K (1-3) cDNA was amplified by PCR, cloned into the vector pGEM-3Zf, and sequenced according to Dye primer sequencing kit.
Results: The functional fragment of angiostatin K (1-3) cDNA (859 bp) was obtained by PCR and determined by sequencing.
Conclusion: The angiostatin K (1-3) gene has been cloned. The gene is of great significance in tumor-antiangiogenesis therapy.