Objective: To establish a method of detecting and localizing newly cloned gene by FISH using short DNA fragment.
Methods: Bio-14-dATP was incorporated into the target gene cloned in plasmid by nick translation followed by conventional FISH with some modification. By Means of this method, c-met, c-erbB2, 1A6, APC and Rb genes were examined in ovarian and gastric cancer cell lines.
Results: 1A6, a newly isolated gene was located at the long arm of one of the C group chromosomes. Oncogene c-met, c-erbB2 and trmor suppressor gene APC, Rb were shown to produce clear-cut hybridization signals in ovarian and gastric cancer cells.
Conclusion: Short DNA fragment cloned in plasmid is useful for studies of gene localization, gene deletion and amplification by FISH.