Dihydropyridine neuropeptide Y Y(1) receptor antagonists

Bioorg Med Chem Lett. 2002 Feb 11;12(3):379-82. doi: 10.1016/s0960-894x(01)00761-2.

Abstract

Dihydropyridine 5a was found to be an inhibitor of neuropeptide Y(1) binding in a high throughput (125)I-PYY screening assay. Structure-activity studies around certain portions of the dihydropyridine chemotype identified BMS-193885 (6e) as a potent and selective Y(1) receptor antagonist. In a forskolin-stimulated c-AMP production assay using CHO cells expressing the human Y(1) receptor, 6e demonstrated full functional antagonism (K(b)=4.5 nM). Compound 6e inhibited NPY-induced feeding in satiated rats when dosed at 3.0 and 10.0 mg/kg (ip), and also decreased spontaneous overnight food consumption in rats at doses of 10 and 20 mg/kg (ip).

MeSH terms

  • Animals
  • Anti-Obesity Agents / chemical synthesis*
  • Anti-Obesity Agents / pharmacology*
  • CHO Cells
  • Cell Line
  • Cell Membrane / drug effects
  • Cell Membrane / metabolism
  • Cricetinae
  • Dihydropyridines / chemical synthesis*
  • Dihydropyridines / pharmacology*
  • Eating / drug effects
  • Humans
  • Kinetics
  • Phenylurea Compounds / chemical synthesis*
  • Phenylurea Compounds / pharmacology*
  • Radioligand Assay
  • Rats
  • Receptors, Neuropeptide Y / antagonists & inhibitors*
  • Structure-Activity Relationship

Substances

  • Anti-Obesity Agents
  • BMS 193885
  • Dihydropyridines
  • Phenylurea Compounds
  • Receptors, Neuropeptide Y
  • neuropeptide Y-Y1 receptor