The objective of the study was to measure the level of HIV/AIDS related anxiety among hospital nursing staff and identify its determinants. Data were obtained through anonymous self administered questionnaire distributed to 222 nurses. The rasge anxiety and the HIV/AIDS attitudes were the most determinants of the anxiety level in the participants. Specifically, negative HIV/AIDS attitudes was associated with raise in state anxiety level, as well as high level of rasge anxiety was associated with high level of state anxiety. In conclusion, it is necessary to provide to the participants update trainings that included different aspects of HIV infection, as well as, anxiety management when caring for persons with HIV/AIDS.