Generation of minimal protein identifiers of proteins from two-dimensional gels and recombinant proteins

Frank Schmidt; Angelika Lueking; Eckhard Nordhoff; Johan Gobom; Joachim Klose; Harald Seitz; Volker Egelhofer; Holger Eickhoff; Hans Lehrach; Dolores J Cahill

doi:10.1002/1522-2683(200202)23:4<621::AID-ELPS621>3.0.CO;2-J

Generation of minimal protein identifiers of proteins from two-dimensional gels and recombinant proteins

Electrophoresis. 2002 Feb;23(4):621-5. doi: 10.1002/1522-2683(200202)23:4<621::AID-ELPS621>3.0.CO;2-J.

Authors

Frank Schmidt¹, Angelika Lueking, Eckhard Nordhoff, Johan Gobom, Joachim Klose, Harald Seitz, Volker Egelhofer, Holger Eickhoff, Hans Lehrach, Dolores J Cahill

Affiliation

¹ Max Planck Institute for Molecular Genetics, Berlin, Germany.

PMID: 11870774
DOI: 10.1002/1522-2683(200202)23:4<621::AID-ELPS621>3.0.CO;2-J

Abstract

We describe the technical feasibility and methodology to characterize a protein by a minimal set of structural information generated by matrix assisted laser desorption/ionization (MALDI)-mass spectrometry, termed a "minimal protein Identifier" (MPI). MPIs can be determined for proteins from two-dimensional gels and recombinant proteins and can be used to compare and identify proteins from these sources.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Brain / embryology
Cloning, Molecular
Databases, Protein
Electrophoresis, Gel, Two-Dimensional / methods*
Fetus
Humans
Peptide Fragments / analysis
Peptide Library
Proteins / analysis*
Proteome / analysis
Recombinant Proteins / analysis
Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / methods*

Substances

Peptide Fragments
Peptide Library
Proteins
Proteome
Recombinant Proteins