Abstract
Comparison of the whole-genome sequence of Bacillus anthracis isolated from a victim of a recent bioterrorist anthrax attack with a reference reveals 60 new markers that include single nucleotide polymorphisms (SNPs), inserted or deleted sequences, and tandem repeats. Genome comparison detected four high-quality SNPs between the two sequenced B. anthracis chromosomes and seven differences among different preparations of the reference genome. These markers have been tested on a collection of anthrax isolates and were found to divide these samples into distinct families. These results demonstrate that genome-based analysis of microbial pathogens will provide a powerful new tool for investigation of infectious disease outbreaks.
Publication types
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Comparative Study
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Research Support, Non-U.S. Gov't
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Research Support, U.S. Gov't, Non-P.H.S.
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Animals
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Anthrax / microbiology
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Bacillus anthracis / classification
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Bacillus anthracis / genetics*
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Bacillus anthracis / isolation & purification
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Bacillus anthracis / pathogenicity
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Bacterial Typing Techniques
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Base Sequence
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Bioterrorism
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Chromosome Inversion
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Computational Biology
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Disease Outbreaks
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Genetic Markers
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Genetic Variation*
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Genome, Bacterial*
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Genomics
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Humans
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Minisatellite Repeats
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Molecular Sequence Data
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Mutation
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Phenotype
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Phylogeny
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Plasmids
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Polymorphism, Single Nucleotide*
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Recombination, Genetic
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Repetitive Sequences, Nucleic Acid
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Sequence Analysis, DNA*
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Sequence Deletion
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Species Specificity
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Transposases / genetics
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Virulence / genetics
Substances
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Genetic Markers
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Transposases
Associated data
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GENBANK/AE011190
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GENBANK/AE011191