Abstract
A total of 2,718 blood samples were analyzed in five virological laboratories for the presence of cytomegalovirus (CMV) by in-house tests and one standardized plasma PCR assay. Results from in-house tests showed remarkable variability. Detection of CMV pp65 antigen or DNA from cells was more sensitive than that by plasma CMV PCR assay.
Publication types
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Evaluation Study
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Research Support, Non-U.S. Gov't
MeSH terms
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Culture Media
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Cytomegalovirus / genetics
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Cytomegalovirus / isolation & purification*
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Cytomegalovirus Infections / diagnosis
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Cytomegalovirus Infections / virology
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DNA, Viral / blood*
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Hematopoietic Stem Cell Transplantation / adverse effects
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Hospitals, University
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Humans
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Laboratories, Hospital*
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Observer Variation
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Phosphoproteins / blood*
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Polymerase Chain Reaction / methods
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Polymerase Chain Reaction / standards
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Reproducibility of Results
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Viral Matrix Proteins / blood*
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Virology*
Substances
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Culture Media
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DNA, Viral
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Phosphoproteins
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Viral Matrix Proteins
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cytomegalovirus matrix protein 65kDa