Abstract
Through differential killer cell Ig-like receptor (KIR) and CD94:NKG2 gene expression, human NK cells generate diverse repertoires, each cell having an inhibitory receptor for autologous HLA class I. Using a new method for measuring repertoire difference that integrates multiple flow cytometry parameters, we found individual repertoire stability, but population variability. Correlating repertoire differences with KIR and HLA genotype for 85 sibling pairs reveals the dominant influence of KIR genotype; HLA genotype having a subtle, modulating effect on relative KIR expression frequencies. HLA and/or KIR genotype also influences CD94:NKG2A expression. After HLA-matched stem cell transplantation, KIR repertoires either recapitulated that of the donor or were generally depressed for KIR expression. Human NK cell repertoires are defined by combinations of variable KIR and HLA class I genes and conserved CD94:NKG2 genes.
Publication types
-
Comparative Study
-
Research Support, Non-U.S. Gov't
-
Research Support, U.S. Gov't, P.H.S.
MeSH terms
-
Adult
-
Aged
-
Antigens, CD / biosynthesis
-
Antigens, CD / genetics
-
Clone Cells
-
Flow Cytometry
-
Gene Expression Regulation / immunology*
-
Genotype
-
Hematopoietic Stem Cell Transplantation
-
Histocompatibility Testing
-
Humans
-
Killer Cells, Natural / immunology*
-
Killer Cells, Natural / metabolism*
-
Lectins, C-Type*
-
Lymphocyte Count
-
Lymphocyte Subsets / immunology*
-
Lymphocyte Subsets / metabolism*
-
Male
-
Membrane Glycoproteins / biosynthesis
-
Membrane Glycoproteins / genetics
-
Middle Aged
-
NK Cell Lectin-Like Receptor Subfamily C
-
NK Cell Lectin-Like Receptor Subfamily D
-
Nuclear Family
-
Receptors, Immunologic / biosynthesis
-
Receptors, Immunologic / genetics*
-
Receptors, Immunologic / metabolism
-
Receptors, KIR
-
Receptors, Natural Killer Cell
Substances
-
Antigens, CD
-
KLRC1 protein, human
-
KLRD1 protein, human
-
Lectins, C-Type
-
Membrane Glycoproteins
-
NK Cell Lectin-Like Receptor Subfamily C
-
NK Cell Lectin-Like Receptor Subfamily D
-
Receptors, Immunologic
-
Receptors, KIR
-
Receptors, Natural Killer Cell