Effects of human antithymocyte globulin on acetylcholine synthesis, its release and choline acetyltransferase transcription in a human leukemic T-cell line

J Neuroimmunol. 2002 Jul;128(1-2):1-8. doi: 10.1016/s0165-5728(02)00111-x.

Abstract

Lymphocytes possess an independent, nonneuronal cholinergic system. In the present study, we investigated the short- and long-term effects of antithymocyte globulin (ATG)-Fresenius (ATG-F), a human antithymocyte globulin that binds to CD2, CD7 and CD11a, on acetylcholine (ACh) synthesis and transcription of choline acetyltransferase (ChAT) in CCRF-CEM cells, a human leukemic T-cell line. In the short-term (6 h), ATG-F enhanced ACh release, likely through transient increases in intracellular Ca(2+) ([Ca(2+)](i)) mediated by CD7, which led to declines in intracellular ACh content. By 48 h, however, the ACh content had increased as compared to control due to up-regulation of ChAT expression mediated by CD11a.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Acetylcholine / biosynthesis*
  • Acetylcholine / metabolism
  • Antigens, CD7 / immunology
  • Antigens, CD7 / metabolism
  • Antigens, Surface / drug effects
  • Antigens, Surface / immunology*
  • Antigens, Surface / metabolism
  • Antilymphocyte Serum / immunology*
  • Antilymphocyte Serum / pharmacology
  • CD11 Antigens / immunology
  • CD11 Antigens / metabolism
  • Calcium / metabolism*
  • Calcium Signaling / drug effects
  • Calcium Signaling / immunology
  • Cell Division / drug effects
  • Cell Division / immunology
  • Choline O-Acetyltransferase / genetics
  • Choline O-Acetyltransferase / metabolism*
  • Humans
  • Intracellular Fluid / drug effects
  • Intracellular Fluid / immunology
  • Intracellular Fluid / metabolism
  • Leukemia
  • RNA, Messenger / drug effects
  • RNA, Messenger / metabolism
  • Signal Transduction / drug effects
  • Signal Transduction / immunology*
  • T-Lymphocytes / drug effects
  • T-Lymphocytes / immunology*
  • T-Lymphocytes / metabolism*
  • Transcription, Genetic / drug effects
  • Transcription, Genetic / immunology
  • Tumor Cells, Cultured
  • Up-Regulation / drug effects
  • Up-Regulation / immunology

Substances

  • Antigens, CD7
  • Antigens, Surface
  • Antilymphocyte Serum
  • CD11 Antigens
  • RNA, Messenger
  • Choline O-Acetyltransferase
  • Acetylcholine
  • Calcium