Silica-based artificial protease exploiting aldehyde groups as catalytic elements

Bioorg Med Chem Lett. 2002 Sep 16;12(18):2557-60. doi: 10.1016/s0960-894x(02)00484-5.

Abstract

An artificial protease synthesized by covering the surface of silica gel with aldehyde and indole groups effectively hydrolyzed albumin and gamma-globulin. It is proposed that the aldehyde group is involved in both complexation of the protein substrate and the catalytic cleavage of the peptide bonds of the complexed proteins.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Aldehydes / chemistry*
  • Catalysis
  • Electrophoresis, Polyacrylamide Gel
  • Endopeptidases / chemistry*
  • Hydrolysis
  • Kinetics
  • Silicon Dioxide / chemistry*

Substances

  • Aldehydes
  • Silicon Dioxide
  • Endopeptidases