Abstract
Micro-RNAs (miR genes) are a large family of highly conserved noncoding genes thought to be involved in temporal and tissue-specific gene regulation. MiRs are transcribed as short hairpin precursors ( approximately 70 nt) and are processed into active 21- to 22-nt RNAs by Dicer, a ribonuclease that recognizes target mRNAs via base-pairing interactions. Here we show that miR15 and miR16 are located at chromosome 13q14, a region deleted in more than half of B cell chronic lymphocytic leukemias (B-CLL). Detailed deletion and expression analysis shows that miR15 and miR16 are located within a 30-kb region of loss in CLL, and that both genes are deleted or down-regulated in the majority ( approximately 68%) of CLL cases.
Publication types
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Comparative Study
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Research Support, Non-U.S. Gov't
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Adult
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B-Lymphocytes / chemistry
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B-Lymphocytes / cytology
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Blotting, Northern
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Blotting, Western
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CD5 Antigens / analysis
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Child
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Child, Preschool
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Chromosomes, Human, Pair 13 / genetics*
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Gene Deletion*
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Gene Expression Regulation, Leukemic*
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Humans
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Hybrid Cells / chemistry
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Leukemia, Lymphocytic, Chronic, B-Cell / genetics*
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MicroRNAs / biosynthesis
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MicroRNAs / genetics*
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Middle Aged
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Neoplasms / genetics
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Neoplasms / pathology
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Neoplastic Stem Cells / chemistry
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Palatine Tonsil / cytology
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RNA, Neoplasm / biosynthesis
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RNA, Neoplasm / genetics*
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Reverse Transcriptase Polymerase Chain Reaction
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Transcription, Genetic
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Tumor Cells, Cultured / chemistry
Substances
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CD5 Antigens
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MicroRNAs
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RNA, Neoplasm