Abstract
CodY is a GTP sensor that represses transcription of early stationary phase and sporulation genes in Bacillus subtilis. As nutrients become limiting, GTP levels fall and CodY-mediated repression is relieved. Crystals of CodY have been grown in the presence and absence of GTP from sodium citrate buffered solutions containing lithium sulfate and diffraction data have been collected extending to 3.5 A spacing.
Publication types
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Research Support, Non-U.S. Gov't
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Bacillus subtilis / chemistry*
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Bacillus subtilis / genetics
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Bacterial Proteins / biosynthesis
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Bacterial Proteins / chemistry*
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Bacterial Proteins / genetics
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Bacterial Proteins / metabolism
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Crystallization / methods
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Crystallography, X-Ray
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DNA Primers / genetics
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DNA-Binding Proteins / biosynthesis
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DNA-Binding Proteins / chemistry*
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DNA-Binding Proteins / genetics
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DNA-Binding Proteins / metabolism
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Escherichia coli / genetics
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Escherichia coli / metabolism
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Guanosine Triphosphate / metabolism
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Recombinant Proteins / biosynthesis
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Recombinant Proteins / chemistry
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Recombinant Proteins / genetics
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Repressor Proteins / biosynthesis
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Repressor Proteins / chemistry*
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Repressor Proteins / genetics
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Repressor Proteins / metabolism
Substances
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Bacterial Proteins
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DNA Primers
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DNA-Binding Proteins
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Recombinant Proteins
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Repressor Proteins
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Guanosine Triphosphate