Abstract
RNA interference (RNAi) is a conserved mechanism in which double-stranded, small interfering RNAs (siRNAs) trigger a sequence-specific gene-silencing process. Here we describe the inhibition of murine herpesvirus 68 replication by siRNAs targeted to sequences encoding Rta, an immediate-early protein known as an initiator of the lytic viral gene expression program, and open reading frame 45 (ORF 45), a conserved viral protein. Our results suggest that RNAi can block gammaherpesvirus replication and ORF 45 is required for efficient viral production.
Publication types
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Research Support, Non-U.S. Gov't
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Animals
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Cell Line
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Cricetinae
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Gammaherpesvirinae / drug effects
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Gammaherpesvirinae / physiology*
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Gene Expression Regulation, Viral
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Humans
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Immediate-Early Proteins / genetics
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Immediate-Early Proteins / metabolism
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Mice
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Open Reading Frames / genetics
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Open Reading Frames / physiology
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RNA Interference*
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RNA, Small Interfering / pharmacology*
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Viral Proteins / genetics
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Viral Proteins / metabolism
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Virus Replication / drug effects*
Substances
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Immediate-Early Proteins
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RNA, Small Interfering
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Viral Proteins