Abstract
We compared light-induced fluorescence (LIF) to nominal injected drug dose for predicting the depth of necrosis response to photodynamic therapy (PDT) in a murine tumor model. Mice were implanted with radiation-induced fibrosarcoma (RIF) and were injected with 0, 5, or 10 mg/kg Photofrin. 630-nm light (30 J/cm(2), 75 mW/cm(2)) was delivered to the tumor after 24 hours. Fluorescence emission (lambda(excitation)=545 nm, lambda( emission)=628 nm) from the tumor was measured. The LIF data had less scatter than injected drug dose, and was found to be at least as good as an injected drug dose for predicting the depth of necrosis after PDT. Our observations provide further evidence that fluorescence spectroscopy can be used to quantify tissue photosensitizer uptake and to predict PDT tissue damage.
Publication types
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Comparative Study
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Evaluation Study
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Research Support, U.S. Gov't, P.H.S.
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Validation Study
MeSH terms
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Animals
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Antineoplastic Agents / administration & dosage
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Antineoplastic Agents / pharmacokinetics
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Dihematoporphyrin Ether / administration & dosage*
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Dihematoporphyrin Ether / pharmacokinetics*
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Dose-Response Relationship, Drug
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Female
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Fibrosarcoma / diagnosis
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Fibrosarcoma / drug therapy
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Fibrosarcoma / metabolism*
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Fibrosarcoma / pathology*
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Injections, Intravenous
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Mice
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Mice, Inbred C3H
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Necrosis
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Neoplasm Transplantation
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Neoplasms, Radiation-Induced
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Photochemotherapy / methods*
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Photosensitizing Agents / administration & dosage
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Photosensitizing Agents / pharmacokinetics
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Predictive Value of Tests
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Spectrometry, Fluorescence / methods*
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Statistics as Topic
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Tissue Distribution
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Treatment Outcome
Substances
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Antineoplastic Agents
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Photosensitizing Agents
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Dihematoporphyrin Ether