Stress-induced mutagenesis in bacteria

Science. 2003 May 30;300(5624):1404-9. doi: 10.1126/science.1082240.

Abstract

The evolutionary significance of stress-induced mutagenesis was evaluated by studying mutagenesis in aging colonies (MAC) of Escherichia coli natural isolates. A large fraction of isolates exhibited a strong MAC, and the high MAC variability reflected the diversity of selective pressures in ecological niches. MAC depends on starvation, oxygen, and RpoS and adenosine 3',5'-monophosphate regulons; thus it may be a by-product of genetic strategies for improving survival under stress. MAC could also be selected through beneficial mutations that it generates, as shown by computer modeling and the patterns of stress-inducible and constitutive mutagenesis. We suggest that irrespective of the causes of their emergence, stress-induced mutations participate in adaptive evolution.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adaptation, Physiological
  • Air Microbiology
  • Alleles
  • Animals
  • Bacterial Proteins / genetics
  • Bacterial Proteins / metabolism
  • Biological Evolution*
  • Computer Simulation
  • DNA Repair
  • DNA-Directed RNA Polymerases / genetics
  • Escherichia coli / classification
  • Escherichia coli / genetics*
  • Escherichia coli / growth & development
  • Escherichia coli / physiology*
  • Escherichia coli Infections / microbiology
  • Escherichia coli Proteins / genetics
  • Escherichia coli Proteins / metabolism
  • Feces / microbiology
  • Gene Expression Regulation, Bacterial
  • Genes, Bacterial
  • Geologic Sediments
  • Humans
  • Lac Operon
  • Models, Biological
  • Mutagenesis*
  • Oxidative Stress
  • Oxygen / metabolism
  • Phenotype
  • Phylogeny
  • Rec A Recombinases / metabolism
  • Regulon
  • SOS Response, Genetics
  • Selection, Genetic
  • Sigma Factor / genetics
  • Sigma Factor / metabolism
  • Water Microbiology

Substances

  • Bacterial Proteins
  • Escherichia coli Proteins
  • Sigma Factor
  • sigma factor KatF protein, Bacteria
  • Rec A Recombinases
  • DNA-Directed RNA Polymerases
  • RNA polymerase beta subunit
  • Oxygen